| Title: | [Development of new SSR markers from EST of SSH cDNA libraries on rose fragrance] |
| Author(s): | Yan HJ; Zhang H; Xie JR; Li SF; Jian HY; Qiu XQ; Wang QG; Wang JH; Tang KX; |
| Address: | "Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China. hjyan8203@126.com" |
| DOI: | 10.3724/sp.j.1005.2009.00962 |
| ISSN/ISBN: | 0253-9772 (Print) 0253-9772 (Linking) |
| Abstract: | "The new SSR markers of rose related fragrance were developed based on the SSH cDNA libraries of rose floral scent mutant. In this study, 10 EST-SSRs (2.6%) from 391 ESTs in the libraries were identified. Six EST-SSRs primers were designed to sequence flanking SSRs. The primer pairs designed were screened on the wild-type Jinyindao, which has flowers full of pleasant scent, and the mutant-type Wangriqinghuai without perceivable floral scent. Five primer pairs were amplified effectively in Jinyindao and Wangriqinghuai, and 3 were polymorphic between Jinyindao and Wangriqinghuai. Eighteen rose cultivars including fragrant roses and nonfragrant roses were identified by the five prime pairs. These results proved that EST-SSR markers are effective markers to identify the polymorphism of the rose" |
| Keywords: | *Expressed Sequence Tags *Gene Library Genetic Markers *Odorants Rosa/*genetics Tandem Repeat Sequences/*genetics; |
| Notes: | "MedlineYan, Hui-Jun Zhang, Hao Xie, Ji-Rong Li, Shu-Fa Jian, Hong-Ying Qiu, Xian-Qin Wang, Qi-Gang Wang, Ji-Hua Tang, Kai-Xue chi China 2009/10/13 Yi Chuan. 2009 Sep; 31(9):962-6. doi: 10.3724/sp.j.1005.2009.00962" |
