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Methods Mol Biol

Title:		Insect pheromone behavior: fruit fly
Author(s):		Yamamoto D; Kohatsu S; Koganezawa M;
Address:		"Department of Developmental Biology and Neurosciences, Tohoku University Graduate School of Life Sciences, Sendai, Japan"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		261 - 272
DOI:		10.1007/978-1-62703-619-1_19
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca(2+)-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays"
Keywords:		"Animals *Courtship Drosophila Proteins/genetics/metabolism Drosophila melanogaster/*physiology Female Ion Channels Male Nerve Tissue Proteins/genetics Neurons/cytology/metabolism Pheromones/*metabolism Repressor Proteins/genetics Sexual Behavior, Animal/*;neuroscience;"
Notes:		"MedlineYamamoto, Daisuke Kohatsu, Soh Koganezawa, Masayuki eng Research Support, Non-U.S. Gov't 2013/09/10 Methods Mol Biol. 2013; 1068:261-72. doi: 10.1007/978-1-62703-619-1_19"