| Title: | "Cloning and expression of the gene encoding the diapause hormone and pheromone biosynthesis activating neuropeptide of the beet armyworm, Spodoptera exigua" |
| Author(s): | Xu J; Su JY; Shen JL; Xu WH; |
| Address: | "Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, People's Republic of China" |
| DOI: | 10.1080/10425170601060806 |
| ISSN/ISBN: | 1042-5179 (Print) 1026-7913 (Linking) |
| Abstract: | "Diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN), two important insect neuropeptides, regulate insect development and sex pheromone biosynthesis, respectively. DH-like immunoreactivity has been detected in the suboesophageal ganglion (SG) of pharate adult of Spodoptera exigua (Spe) by using an antiserum against Helicoverpa armigera DH. A full-length of Spe-DH-PBAN cDNA was obtained based on reverse transcription-PCR and rapid amplification of cDNA ends strategies. The open reading frame of this cDNA encodes a 197-amino acid precursor protein that contains DH, PBAN, and three other SG neuropeptides, all of which share a conservative C-terminal pentapeptide motif FXPR/KL (X = G, T or S). Northern blot analysis demonstrates the presence of an 800 bp transcript in the SG. The Spe-DH-PBAN mRNA is detectable at high levels at larval and adult stages, suggesting that Spe-DH-PBAN gene might be correlated with larval development and sex pheromone biosynthesis in moths" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary/genetics Immunohistochemistry Insect Proteins/*genetics/metabolism Molecular Sequence Data Neuropeptides/*genetics/metabolism Phylogeny Sequence Homology, Amino Acid Spodopter;" |
| Notes: | "MedlineXu, Jun Su, Jian-Ya Shen, Jin-Liang Xu, Wei-Hua eng Research Support, Non-U.S. Gov't England 2007/03/17 DNA Seq. 2007 Apr; 18(2):145-51. doi: 10.1080/10425170601060806" |
