| Title: | Identification of Pseudomonas strains for the biological control of soybean red crown root rot |
| Author(s): | Win KT; Kobayashi M; Tanaka F; Takeuchi K; Oo AZ; Jiang CJ; |
| Address: | "Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, 305-8602, Japan. Research Center for Advanced Analysis, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki, 3058686, Japan. Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, 305-8602, Japan. cypa44@hotmail.com. Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. cypa44@hotmail.com" |
| DOI: | 10.1038/s41598-022-18905-2 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Soybean red crown root rot (RCR), caused by the soil-borne fungal pathogen, Calonectria ilicicola, is the most destructive disease affecting soybean production in Japan. To date, no resistant cultivars or effective fungicides have been developed to control this disease. In this study, we evaluated 13 bacterial strains to determine their efficacy in controlling C. ilicicola. We first investigated whether the volatile organic compounds (VOCs) emitted by the bacterial strains exhibited any antifungal activity against C. ilicicola using the double-plate chamber method. The results showed that VOCs from three Pseudomonas bacterial strains, OFT2 (Pseudomonas sp.), OFT5 (Pseudomonas sp.), and Cab57 (Pseudomonas protegens), exhibited strong inhibitory activity against C. ilicicola mycelial growth. Some antifungal activity was also observed in the culture supernatants of these Pseudomonas strains. Greenhouse soil inoculation tests showed that application of OFT2, OFT5, and Cab57 cultures around soybean seeds after seed sowing significantly reduced the severity of RCR, as shown by up to 40% reduction in C. ilicicola fungal growth in the roots and 180-200% increase in shoot and root fresh weights compared to the water control. Our results suggest that OFT2, Cab57, and OFT5 produce potent antifungal compounds against C. ilicicola, thereby showing considerable potential for the biological control of C. ilicicola during soybean production" |
| Keywords: | *Antifungal Agents/pharmacology Pseudomonas Seeds Soil *Soybeans/microbiology; |
| Notes: | "MedlineWin, Khin Thuzar Kobayashi, Michie Tanaka, Fukuyo Takeuchi, Kasumi Oo, Aung Zaw Jiang, Chang-Jie eng Research Support, Non-U.S. Gov't England 2022/08/26 Sci Rep. 2022 Aug 25; 12(1):14510. doi: 10.1038/s41598-022-18905-2" |
