| Title: | Breath analysis by two-dimensional gas chromatography with dual flame ionisation and mass spectrometric detection - Method optimisation and integration within a large-scale clinical study |
| Author(s): | Wilde MJ; Cordell RL; Salman D; Zhao B; Ibrahim W; Bryant L; Ruszkiewicz D; Singapuri A; Free RC; Gaillard EA; Beardsmore C; Thomas CLP; Brightling CE; Siddiqui S; Monks PS; |
| Address: | "Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK. Centre of Analytical Science, Loughborough University, Epinal Way, Loughborough, LE11 3TU, UK. Leicester NIHR Biomedical Research Centre (Respiratory theme), Glenfield Hospital, Groby Road, Leicester, LE3 9QP, UK. Leicester NIHR Biomedical Research Centre (Respiratory theme), Glenfield Hospital, Groby Road, Leicester, LE3 9QP, UK; College of Life Sciences, Department of Infection, Immunity and Inflammation, University of Leicester, University Road, Leicester, LE1 7RH, UK. Paediatric Clinical Investigation Centre, Leicester NIHR Biomedical Research Centre (Respiratory theme), University of Leicester, Leicester Royal Infirmary, Leicester, LE2 7LX, UK. Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK. Electronic address: paul.monks@leicester.ac.uk" |
| DOI: | 10.1016/j.chroma.2019.02.001 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Print) 0021-9673 (Linking) |
| Abstract: | "Precision medicine has spurred new innovations in molecular pathology leading to recent advances in the analysis of exhaled breath as a non-invasive diagnostic tool. Volatile organic compounds (VOCs) detected in exhaled breath have the potential to reveal a wealth of chemical and metabolomic information. This study describes the development of a method for the analysis of breath, based on automated thermal desorption (TD) combined with flow modulated comprehensive two-dimensional gas chromatography (GCxGC) with dual flame ionisation and quadrupole mass spectrometric detection (FID and qMS). The constrained optimisation and analytical protocol was designed to meet the practical demands of a large-scale multi-site clinical study, while maintaining analytical rigour to produce high fidelity data. The results demonstrate a comprehensive method optimisation for the collection and analysis of breath VOCs by GCxGC, integral to the standardisation and integration of breath analysis within large clinical studies" |
| Keywords: | Breath Tests/*methods Clinical Studies as Topic/*methods *Flame Ionization *Gas Chromatography-Mass Spectrometry Humans Reference Standards Volatile Organic Compounds/*analysis Breath analysis Comprehensive two-dimensional gas chromatography Flow modulati; |
| Notes: | "MedlineWilde, Michael J Cordell, Rebecca L Salman, Dahlia Zhao, Bo Ibrahim, Wadah Bryant, Luke Ruszkiewicz, Dorota Singapuri, Amisha Free, Robert C Gaillard, Erol A Beardsmore, Caroline Thomas, C L Paul Brightling, Chris E Siddiqui, Salman Monks, Paul S eng MR/N005880/1/MRC_/Medical Research Council/United Kingdom Netherlands 2019/02/14 J Chromatogr A. 2019 Jun 7; 1594:160-172. doi: 10.1016/j.chroma.2019.02.001. Epub 2019 Feb 5" |
