| Title: | Functions of stone cells and oleoresin terpenes in the conifer defense syndrome |
| Author(s): | Whitehill JGA; Yuen MMS; Henderson H; Madilao L; Kshatriya K; Bryan J; Jaquish B; Bohlmann J; |
| Address: | "Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, V6T 1Z4, Canada. Department of Statistics, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. British Columbia Ministry of Forests, Lands and Natural Resource Operations, Tree Improvement Branch, Kalamalka Forestry Centre, 3401 Reservoir Road, Vernon, BC, V1B 2C7, Canada. Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC, V6T 1Z4, Canada. Department of Forest and Conservation Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "Conifers depend on complex defense systems against herbivores. Stone cells (SC) and oleoresin are physical and chemical defenses of Sitka spruce that have been separately studied in previous work. Weevil oviposit at the tip of the previous year's apical shoot (PYAS). We investigated interactions between weevil larvae and trees in controlled oviposition experiments with resistant (R) and susceptible (S) Sitka spruce. R trees have an abundance of SC in the PYAS cortex. SC are mostly absent in S trees. R trees and S trees also differ in the composition of oleoresin terpenes. Transcriptomes of R and S trees revealed differences in long-term weevil-induced responses. Performance of larvae was significantly reduced on R trees compared with S trees under experimental conditions that mimicked natural oviposition behavior at apical shoot tips and may be attributed to the effects of SC. In oviposition experiments designed for larvae to feed below the area of highest SC abundance, larvae showed an unusual feeding behavior and oleoresin appeared to function as the major defense. The results support a role for both SC and oleoresin terpenes and possible synergies between these traits in the defense syndrome of weevil-resistant Sitka spruce" |
| Keywords: | "Animals Feeding Behavior Gastrointestinal Tract/physiology Gene Expression Regulation, Plant Larva/physiology Oviposition Plant Extracts/*metabolism Terpenes/*metabolism Tracheophyta/*cytology/genetics/*metabolism Transcriptome/genetics Trees/genetics/phy;" |
| Notes: | "MedlineWhitehill, Justin G A Yuen, Macaire M S Henderson, Hannah Madilao, Lina Kshatriya, Kristina Bryan, Jennifer Jaquish, Barry Bohlmann, Jorg eng Genome Quebec/International Natural Sciences and Engineering Research Council of Canada/International Genome Canada/International Research Support, Non-U.S. Gov't England 2018/09/15 New Phytol. 2019 Feb; 221(3):1503-1517. doi: 10.1111/nph.15477. Epub 2018 Oct 23" |
