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PLoS One

Title:		Detection of plant volatiles after leaf wounding and darkening by proton transfer reaction 'time-of-flight' mass spectrometry (PTR-TOF)
Author(s):		Brilli F; Ruuskanen TM; Schnitzhofer R; Muller M; Breitenlechner M; Bittner V; Wohlfahrt G; Loreto F; Hansel A;
Address:		"Ionicon Analytik G.m.b.H., Innsbruck, Austria"
Journal Title:		PLoS One
Year:		2011
Volume:		20110526
Issue:		5
Page Number:		e20419 -
DOI:		10.1371/journal.pone.0020419
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Proton transfer reaction-time of flight (PTR-TOF) mass spectrometry was used to improve detection of biogenic volatiles organic compounds (BVOCs) induced by leaf wounding and darkening. PTR-TOF measurements unambiguously captured the kinetic of the large emissions of green leaf volatiles (GLVs) and acetaldehyde after wounding and darkening. GLVs emission correlated with the extent of wounding, thus confirming to be an excellent indicator of mechanical damage. Transient emissions of methanol, C5 compounds and isoprene from plant species that do not emit isoprene constitutively were also detected after wounding. In the strong isoprene-emitter Populus alba, light-dependent isoprene emission was sustained and even enhanced for hours after photosynthesis inhibition due to leaf cutting. Thus isoprene emission can uncouple from photosynthesis and may occur even after cutting leaves or branches, e.g., by agricultural practices or because of abiotic and biotic stresses. This observation may have important implications for assessments of isoprene sources and budget in the atmosphere, and consequences for tropospheric chemistry"
Keywords:		Carbon/analysis Citrus/chemistry Dactylis/chemistry *Darkness Gases/analysis Ions Kinetics Mass Spectrometry/*methods Plant Leaves/*chemistry Plants/*chemistry Populus/chemistry *Protons Terpenes/analysis Time Factors Volatile Organic Compounds/*analysis/;
Notes:		"MedlineBrilli, Federico Ruuskanen, Taina M Schnitzhofer, Ralf Muller, Markus Breitenlechner, Martin Bittner, Vinzenz Wohlfahrt, Georg Loreto, Francesco Hansel, Armin eng P 19849/FWF_/Austrian Science Fund FWF/Austria P 23267/FWF_/Austrian Science Fund FWF/Austria Research Support, Non-U.S. Gov't 2011/06/04 PLoS One. 2011; 6(5):e20419. doi: 10.1371/journal.pone.0020419. Epub 2011 May 26"