| Title: | Identification of Exhaled Metabolites in Children with Cystic Fibrosis |
| Author(s): | Weber R; Perkins N; Bruderer T; Micic S; Moeller A; |
| Address: | "Department of Respiratory Medicine and Childhood Research Center, University Children's Hospital Zurich, Steinwiesstrasse 75, 8032 Zurich, Switzerland. Division of Clinical Chemistry and Biochemistry, University of Zurich, Steinwiesstrasse 75, 8032 Zurich, Switzerland. Department of Chemistry and Industrial Chemistry, University of Pisa, Via Giuseppe Moruzzi 13, 56124 Pisa, Italy. Faculty of Medicine, University of Zurich, Raemistrasse 71, 8006 Zurich, Switzerland" |
| ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
| Abstract: | "The early detection of inflammation and infection is important to prevent irreversible lung damage in cystic fibrosis. Novel and non-invasive monitoring tools would be of high benefit for the quality of life of patients. Our group previously detected over 100 exhaled mass-to-charge (m/z) features, using on-line secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS), which distinguish children with cystic fibrosis from healthy controls. The aim of this study was to annotate as many m/z features as possible with putative chemical structures. Compound identification was performed by applying a rigorous workflow, which included the analysis of on-line MS(2) spectra and a literature comparison. A total of 49 discriminatory exhaled compounds were putatively identified. A group of compounds including glycolic acid, glyceric acid and xanthine were elevated in the cystic fibrosis group. A large group of acylcarnitines and aldehydes were found to be decreased in cystic fibrosis. The proposed compound identification workflow was used to identify signatures of volatile organic compounds that discriminate children with cystic fibrosis from healthy controls, which is the first step for future non-invasive and personalized applications" |
| Keywords: | Sesi-hrms breath analysis children cystic fibrosis infection inflammation putative compound identification; |
| Notes: | "PubMed-not-MEDLINEWeber, Ronja Perkins, Nathan Bruderer, Tobias Micic, Srdjan Moeller, Alexander eng 326030_177101/1/SNSF_/Swiss National Science Foundation/Switzerland Swiss Cystic Fibrosis Foundation/ Evi Diethelm-Winteler Foundation/ Childhood Research Center of the University Children's Hospital Zurich/ Switzerland 2022/10/28 Metabolites. 2022 Oct 17; 12(10):980. doi: 10.3390/metabo12100980" |
