| Title: | Formation of alpha-Farnesene in Tea (Camellia sinensis) Leaves Induced by Herbivore-Derived Wounding and Its Effect on Neighboring Tea Plants |
| Author(s): | Wang X; Zeng L; Liao Y; Li J; Tang J; Yang Z; |
| Address: | "Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China. University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China. Tea Research Institute, Guangdong Academy of Agricultural Sciences & Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Dafeng Road 6, Tianhe District, Guangzhou 510640, China. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China. zyyang@scbg.ac.cn. University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China. zyyang@scbg.ac.cn. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China. zyyang@scbg.ac.cn" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Herbivore-induced plant volatiles (HIPVs) play important ecological roles in defense against stresses. In contrast to model plants, reports on HIPV formation and function in crops are limited. Tea (Camellia sinensis) is an important crop in China. alpha-Farnesene is a common HIPV produced in tea plants in response to different herbivore attacks. In this study, a C. sinensis alpha-farnesene synthase (CsAFS) was isolated, cloned, sequenced, and functionally characterized. The CsAFS recombinant protein produced in Escherichia coli was able to transform farnesyl diphosphate (FPP) into alpha-farnesene and also convert geranyl diphosphate (GPP) to beta-ocimene in vitro. Furthermore, transient expression analysis in Nicotiana benthamiana plants indicated that CsAFS was located in the cytoplasm and could convert FPP to alpha-farnesene in plants. Wounding, to simulate herbivore damage, activated jasmonic acid (JA) formation, which significantly enhanced the CsAFS expression level and alpha-farnesene content. This suggested that herbivore-derived wounding induced alpha-farnesene formation in tea leaves. Furthermore, the emitted alpha-farnesene might act as a signal to activate antibacterial-related factors in neighboring undamaged tea leaves. This research advances our understanding of the formation and signaling roles of common HIPVs in crops such as tea plants" |
| Keywords: | *Herbivory Plant Leaves/*metabolism Plant Physiological Phenomena Sesquiterpenes/*metabolism Signal Transduction Tea/classification/genetics/*metabolism Volatile Organic Compounds/*metabolism Camellis sinensis aroma herbivore jasmonic acid signaling tea v; |
| Notes: | "MedlineWang, Xuewen Zeng, Lanting Liao, Yinyin Li, Jianlong Tang, Jinchi Yang, Ziyin eng 2018M640837/China Postdoctoral Science Foundation/ 2018YFD1000601/National Key Research and Development Program of China/ 2016A030306039/Guangdong Natural Science Foundation for Distinguished Young Scholar/ 2019LM1143/Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams/ Switzerland 2019/08/28 Int J Mol Sci. 2019 Aug 25; 20(17):4151. doi: 10.3390/ijms20174151" |
