« Previous AbstractAssociation of urinary dimethylformamide metabolite with lung function decline: The potential mediating role of systematic inflammation estimated by C-reactive protein    Next AbstractGrazing simplifies soil micro-food webs and decouples their relationships with ecosystem functions in grasslands »

Methods Enzymol

Title:		Effect of OBPs on the response of olfactory receptors
Author(s):		Wang B; Cao S; Liu W; Wang G;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen, China; Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. Electronic address: wangguirong@caas.cn"
Journal Title:		Methods Enzymol
Year:		2020
Volume:		20200522
Issue:
Page Number:		279 - 300
DOI:		10.1016/bs.mie.2020.04.065
ISSN/ISBN:		1557-7988 (Electronic) 0076-6879 (Linking)
Abstract:		"The technique of two-electrode voltage-clamp (TEVC) recording from the heterologous expression system of olfactory receptors (ORs) in Xenopus laevis oocytes has been widely used to deorphanize insect ORs, that is to identify specific ligands for each of them. However, there is a controversial issue on whether ORs are activated by the odorant/OBP complex or the odorant alone. The mechanism of interaction among odorants, odorant-binding proteins (OBPs) and ORs remains largely unknown, due to the limitations in the use of scientific and innovative methods. In this chapter, the modified Xenopus oocytes expression system combined with TEVC technique is used to approach this issue. We describe the experimental strategies and provide detailed protocols for recording the signals generated by ORs in response to odorant/OBP complex at different concentrations. Results obtained by this approach have revealed that the presence of OBPs in the system affects the selectivity and sensitivity responses of ORs. Such studies help understanding the molecular mechanism of odorant detection in peripheral nervous system"
Keywords:		"Animals Insect Proteins Insecta *Receptors, Odorant/genetics Interplay Odorant-binding proteins Olfactory receptors Sex pheromones Two-electrode voltage-clamp recording Xenopus laevis oocytes;"
Notes:		"MedlineWang, Bing Cao, Song Liu, Wei Wang, Guirong eng Research Support, Non-U.S. Gov't 2020/08/24 Methods Enzymol. 2020; 642:279-300. doi: 10.1016/bs.mie.2020.04.065. Epub 2020 May 22"