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Biomol NMR Assign

Title:		"(1)H, (13)C, (15)N resonance assignment of recombinant Euplotes raikovi protein Er-23"
Author(s):		Bowles DP; Hansen AL; Rhoads CA; Mohan S; Yuan C; Magliery TJ;
Address:		"Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, 43210, USA. Department of Chemistry & Biochemistry, The Ohio State University, 100 W. 18th Ave., Columbus, OH, 43210, USA. Campus Chemical Instrument Center, The Ohio State University, 460 W. 12th Ave 209 Biomedical Research Tower, Columbus, OH, 43210, USA. Biophysics Graduate Program, The Ohio State University, Columbus, OH, 43210, USA. Department of Chemistry & Biochemistry, The Ohio State University, 100 W. 18th Ave., Columbus, OH, 43210, USA. magliery.1@osu.edu"
Journal Title:		Biomol NMR Assign
Year:		2018
Volume:		20180606
Issue:		2
Page Number:		291 - 295
DOI:		10.1007/s12104-018-9825-4
ISSN/ISBN:		1874-270X (Electronic) 1874-270X (Linking)
Abstract:		"Er-23 is a small, 51 amino acid, disulfide-rich pheromone protein used for cell signaling by Euplotes raikovi. Ten of the 51 amino acids are cysteine, allowing up to five disulfide bonds. Previous NMR work with Er-23 utilized homologously expressed protein, prohibiting isotopic labeling, and consequently the chemical shift assignments were incomplete. We have expressed uniformly (15)N and (13)C-labeled Er-23 in an E. coli expression system. Here we report the full backbone and side chain resonance assignments for recombinant Er-23"
Keywords:		"Amino Acid Sequence *Nuclear Magnetic Resonance, Biomolecular Pheromones/*chemistry Protozoan Proteins/*chemistry Recombinant Proteins/*chemistry Er-23 Euplotes raikovi Full assignment Nmr Pheromone;"
Notes:		"MedlineBowles, David P Hansen, Alexandar L Rhoads, Calvin A Mohan, Sidharth Yuan, Chunhua Magliery, Thomas J eng R01 GM083114/NH/NIH HHS/ T32 GM008512/NH/NIH HHS/ Research Support, N.I.H., Extramural Netherlands 2018/06/08 Biomol NMR Assign. 2018 Oct; 12(2):291-295. doi: 10.1007/s12104-018-9825-4. Epub 2018 Jun 6"