| Title: | New application of air-drying techniques for studying Ephemeroptera and Plecoptera eggs by scanning electron microscopy |
| Author(s): | Ubero-Pascal N; Fortuno JM; de Los Angeles Puig M; |
| Address: | "Departamento de Zoologia y Antropologia Fisica, Facultad de Biologia, Universidad de Murcia, Campus de Espinardo s/n, 30100 Espinardo-Murcia, Spain. nubero@um.es" |
| ISSN/ISBN: | 1059-910X (Print) 1059-910X (Linking) |
| Abstract: | "Hexamethyldisilizane (HMDS) and tetramethylsilane are organic compounds that are volatile at ambient temperature and which can therefore be used for air-drying biological samples for SEM studies. The techniques using these compounds provide results that are comparable with those obtained by critical point drying, but which involve a very simple process that saves time and money. Both techniques were applied to SEM studies of Ephemeroptera and Plecoptera eggs in order to assess their suitability as alternative methods to critical point drying for these kinds of biological material. The results show no morphological differences between eggs HMDS air-dried and critical point-dried" |
| Keywords: | "Animals Desiccation/*methods Female Histocytological Preparation Techniques/*methods Insecta/growth & development/*ultrastructure Microscopy, Electron, Scanning/methods Organosilicon Compounds Ovum/ultrastructure Silanes Trimethylsilyl Compounds;" |
| Notes: | "MedlineUbero-Pascal, Nicolas Fortuno, Jose Manuel de Los Angeles Puig, Maria eng Evaluation Study Research Support, Non-U.S. Gov't 2005/11/30 Microsc Res Tech. 2005 Dec 1; 68(5):264-71. doi: 10.1002/jemt.20248" |
