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Plant Physiol Biochem

Title:		Microarray analysis of ripening-regulated gene expression and its modulation by 1-MCP and hexanal
Author(s):		Tiwari K; Paliyath G;
Address:		"Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada"
Journal Title:		Plant Physiol Biochem
Year:		2011
Volume:		20110113
Issue:		3
Page Number:		329 - 340
DOI:		10.1016/j.plaphy.2011.01.007
ISSN/ISBN:		1873-2690 (Electronic) 0981-9428 (Linking)
Abstract:		"Hexanal, an inhibitor of phospholipase D, has been successfully applied for the pre- and post-harvest treatment of fruits, vegetables and flowers. Changes in gene expression induced by hexanal and the ethylene antagonist 1-MCP, were analyzed by microarray using TOM2 tomato oligo-array containing approximately 12 000 unigenes. Mature green tomato fruits were treated with 1-MCP and hexanal, RNA isolated after 10 days of storage, and labeled cDNA synthesized for microarray analysis. A large variation in gene expression profile was observed in 1-MCP-treated fruits. Genes for ethylene biosynthetic pathway enzymes such as ACC- synthase/oxidase, ethylene receptor and ethylene response factors were heavily down-regulated in 1-MCP-treated fruits. In addition, genes for key enzymes involved in cell wall degradation and carotenoid development pathways were down-regulated. Hexanal treatment significantly down-regulated ACC-synthase, and to a lesser extent, other components of ethylene signal transduction. By contrast to MCP-treated fruits, hexanal-treated fruits gradually ripened and showed higher levels of lycopene and beta-carotene. GC-MS analysis of volatiles showed a higher level of major volatile components in hexanal-treated fruits. Similarities in the modulation of gene expression by hexanal and 1-MCP suggest that hexanal, in addition to being a PLD inhibitor, may also act as a weak ethylene inhibitor"
Keywords:		"Aldehydes/*pharmacology Carotenoids/genetics/metabolism Cell Wall/metabolism DNA, Complementary/biosynthesis Down-Regulation Enzymes/genetics/metabolism Ethylenes/*antagonists & inhibitors Fruit/*drug effects/growth & development/metabolism Gene Expressio;"
Notes:		"MedlineTiwari, Krishnaraj Paliyath, Gopinadhan eng Research Support, Non-U.S. Gov't France 2011/02/08 Plant Physiol Biochem. 2011 Mar; 49(3):329-40. doi: 10.1016/j.plaphy.2011.01.007. Epub 2011 Jan 13"