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Biosci Rep

Title:		Determination of volatile organic compounds exhaled by cell lines derived from hematological malignancies
Author(s):		Tang H; Lu Y; Zhang L; Wu Z; Hou X; Xia H;
Address:		"Department of Hematology, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei 230022, China. Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, No. 350 Shushanhu Road, Hefei 230031, China. Department of Hematology, The second Affiliated Hospital of Anhui Medical University, No. 678 Furong Road, Hefei 230022, China. Department of Hematology, The Chaohu Hospital of Anhui Medical University, No. 64 Northern Chaohu Road, 238000, China hailongxia123@163.com"
Journal Title:		Biosci Rep
Year:		2017
Volume:		20170621
Issue:		3
Page Number:		-
DOI:		10.1042/BSR20170106
ISSN/ISBN:		1573-4935 (Electronic) 0144-8463 (Print) 0144-8463 (Linking)
Abstract:		"Background: The gas human exhaled contains many volatile organic compounds (VOCs), which is related to the health status of body. Analysis of VOCs has been proposed as a noninvasive diagnostic tool for certain cancers. Detailed research on the VOCs in gas exhaled by cell can characterize cell type specific metabolites and may be helpful to detect the cancer markers in clinical practice.Methods: Solid-phase microextraction (SPME) gas chromatography-mass spectrometry was used to detect VOCs in the headspace of tissue culture flask in non-Hodgkin's lymphoma (NHL) cell line JEKO and acute mononuclear leukemia cell line SHI-1, to elaborate the characteristic gaseous biomarkers of hematological malignancies. While macrophage cells and lymphocytic cells were acted as control. The blank group was only the RPMI 1640 medium containing 10% fetal calf serum that without cells.Results: Comparing with control group, the concentration of dimethyl sulfide, 2,4-dimethylheptane, methylbenzene, o-xylene, dodecane, and 1,3-di-tert-butylbenzene in JEKO cells was relatively higher, while the concentration of ethanol, hexanal, and benzaldehyde was lower. In SHI-1 cells, the levels of 2,4-dimethylheptane, benzene, 4-methyldecane, chloroform, 3,7-dimethyl dodecane, and hexadecane were significantly elevated, but the levels of hexanol and cyclohexanol were distinctly reduced. CONCLUSIONS: This pilot study revealed that the malignant hematological cells could change the components of VOCs in the cell culture flask in a cell type-specific pattern. The traits of VOCs in our setting offered new strategy for hematological malignancies tracing, and would act as potential biomarkers in diagnosis of malignant hematological diseases"
Keywords:		"Analysis of Variance Biomarkers, Tumor/*analysis Cell Line, Tumor Gas Chromatography-Mass Spectrometry Hematologic Neoplasms Humans Leukemia, Monocytic, Acute/*diagnosis/pathology Lymphoma, Non-Hodgkin/*diagnosis/pathology Pilot Projects Solid Phase Micro;"
Notes:		"MedlineTang, Hongxia Lu, Yan Zhang, Lulu Wu, Zhonghui Hou, Xiaofang Xia, Hailong eng England 2017/05/17 Biosci Rep. 2017 Jun 21; 37(3):BSR20170106. doi: 10.1042/BSR20170106. Print 2017 Jun 30"