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Folia Microbiol (Praha)

Title:		Enzymes involved in vinyl acetate decomposition by Pseudomonas fluorescens PCM 2123 strain
Author(s):		Szczyrba E; Gren I; Bartelmus G;
Address:		"Institute of Chemical Engineering, Polish Academy of Sciences, Baltycka 5, 44-100, Gliwice, Poland"
Journal Title:		Folia Microbiol (Praha)
Year:		2014
Volume:		20130803
Issue:		2
Page Number:		99 - 105
DOI:		10.1007/s12223-013-0268-0
ISSN/ISBN:		1874-9356 (Electronic) 0015-5632 (Print) 0015-5632 (Linking)
Abstract:		"Esterases are widely used in food processing industry, but there is little information concerning enzymes involved in decompositions of esters contributing to pollution of environment. Vinyl acetate (an ester of vinyl alcohol and acetic acid) is a representative of volatile organic compounds (VOCs) in decomposition, of which hydrolyses and oxidoreductases are mainly involved. Their activities under periodically changing conditions of environment are essential for the removal of dangerous VOCs. Esterase and alcohol/aldehyde dehydrogenase activities were determined in crude cell extract from Pseudomonas fluorescens PMC 2123 after vinyl acetate induction. All examined enzymes exhibit their highest activity at 30-35 degrees C and pH 7.0-7.5. Esterase preferably hydrolyzed ester bonds with short fatty chains without plain differences for C2 or C4. Comparison of Km values for alcohol and aldehyde dehydrogenases for acetaldehyde suggested that this metabolite was preferentially oxidized than reduced. Activity of alcohol dehydrogenase reducing acetaldehyde to ethanol suggested that one mechanism of defense against the elevated concentration of toxic acetaldehyde could be its temporary reduction to ethanol. Esterase activity was inhibited by phenylmethanesulfonyl fluoride, while beta-mercaptoethanol, dithiothreitol, and ethylenediaminetetraacetic acid had no inhibitor effect. From among metal ions, only Mg(2+) and Fe(2+) stimulated the cleavage of ester bond"
Keywords:		Biotransformation Enzyme Activators/analysis Enzyme Inhibitors/analysis Enzyme Stability Esterases/chemistry/*metabolism Hydrogen-Ion Concentration Kinetics Oxidoreductases/chemistry/*metabolism Pseudomonas fluorescens/*enzymology/*metabolism Temperature;
Notes:		"MedlineSzczyrba, Elzbieta Gren, Izabela Bartelmus, Grazyna eng Research Support, Non-U.S. Gov't 2013/08/06 Folia Microbiol (Praha). 2014 Mar; 59(2):99-105. doi: 10.1007/s12223-013-0268-0. Epub 2013 Aug 3"