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Biochemistry

Title:		Cultures of separated mating types of Blakeslea trispora make D and E forms of trisporic acids
Author(s):		Sutter RP; Dadok J; Bothner-By AA; Smith RR; Mishra PK;
Address:		"Department of Biology, West Virginia University, Morgantown 26506-6057"
Journal Title:		Biochemistry
Year:		1989
Volume:		28
Issue:		9
Page Number:		4060 - 4066
DOI:		10.1021/bi00435a064
ISSN/ISBN:		0006-2960 (Print) 0006-2960 (Linking)
Abstract:		"Trisporic acids are end products of the sex-specific pheromones in mucoraceous fungi. We have found three new trisporic acids in cultures of Blakeslea trispora in which (+) and (-) mating types were separated by a membrane with 0.45-microns pores. Two of the trisporic acids were new compounds; the structure of the third [previously described by Miller and Sutter [(1984) J. Biol. Chem. 259, 6420] as methyl trisporate-E with a hydroxyl group at C-2] was revised. Trisporic acid-E(3R), trisporic acid-E(3S), and trisporic acid-D(2S) were in a 1:1:2 ratio, accounted for 9% of the total trisporic acids, and differed by the position and configuration of a hydroxyl group on the ring at C-2 or C-3, the conformation of the ring, the extent of rotation of the side chain relative to the ring, and either a carbonyl or hydroxyl group on the side chain at C-13. These three compounds accounted for only 0.5% of the total trisporic acids in combined mating type cultures. Since the combined cultures did not metabolize trisporic acid-E(3R), its biosynthesis apparently ceases when opposing mating types contact each other physically. We speculate that B. trispora and Phycomyces blakesleeanus utilize different pheromones to regulate an early event (possibly zygotropism) in sexual development"
Keywords:		"Chromatography, High Pressure Liquid Chromatography, Ion Exchange Crosses, Genetic Fatty Acids, Unsaturated/*biosynthesis/isolation & purification/metabolism Magnetic Resonance Spectroscopy Mass Spectrometry Mucor/metabolism/*physiology;"
Notes:		"MedlineSutter, R P Dadok, J Bothner-By, A A Smith, R R Mishra, P K eng 5 RO1 AI21819-03/AI/NIAID NIH HHS/ RR-00292/RR/NCRR NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1989/05/02 Biochemistry. 1989 May 2; 28(9):4060-6. doi: 10.1021/bi00435a064"