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Biomaterials

Title:		Conversion of monkey fibroblasts to transplantable telencephalic neuroepithelial stem cells
Author(s):		Ai Z; Xiang Z; Li Y; Liu G; Wang H; Zheng Y; Qiu X; Zhao S; Zhu X; Li Y; Ji W; Li T;
Address:		"Yunnan Key Lab of Primate Biomedicine Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, 650500, China. Yunnan Key Lab of Primate Biomedicine Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Chongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology (CAST), Southwest University, No. 1 Tiansheng Road Beibei, Chongqing, 400715, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, 650500, China. Chongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology (CAST), Southwest University, No. 1 Tiansheng Road Beibei, Chongqing, 400715, China. Yunnan Key Lab of Primate Biomedicine Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China. Department of Anesthesiology, No. 157, Jinbi Road, The First People Hospital of Yunnan Province, Kunming, Yunnan, 650032, China. Electronic address: lyh066@gmail.com. Yunnan Key Lab of Primate Biomedicine Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, 650500, China. Electronic address: litq@kbimed.com"
Journal Title:		Biomaterials
Year:		2016
Volume:		20151031
Issue:
Page Number:		53 - 65
DOI:		10.1016/j.biomaterials.2015.10.079
ISSN/ISBN:		1878-5905 (Electronic) 0142-9612 (Linking)
Abstract:		"Non-human primates provide optimal models for the development of stem cell therapies. Although somatic cells have been converted into neural stem/progenitor cells, it is unclear whether telencephalic neuroepithelial stem cells (NESCs) with stable properties can be generated from fibroblasts in primate. Here we report that a combination of transcription factors (Oct4, Sox2, Klf4) with a new culture medium induces rhesus monkey fibroblasts into NESCs, which can develop into miniature neural tube (NT)-like structures at a cell level. Furthermore, single induced NESCs (iNESCs) can generate later-stage 3D-NTs after grown on matrigel in suspension culture. iNESCs express NT cell markers, have a unique gene expression pattern biasing towards telencephalic patterning, and give rise to cortical neurons. Via transplantation, single iNESCs can extensively survive, regenerate myelinated neuron axons and synapse structures in adult monkey striatum and cortex, and differentiate into cortical neurons. Successful transplantation is closely associated with graft regions and grafted cell identities. The ability to generate defined and transplantable iNESCs from primate fibroblasts under a defined condition with predictable fate choices will facilitate disease modeling and cell therapy"
Keywords:		"Animals Cell Lineage/drug effects Cell Transdifferentiation Cells, Cultured Cerebral Cortex/cytology Corpus Striatum/cytology Culture Media/pharmacology Fibroblasts/*cytology/drug effects Genes, Reporter Genetic Vectors/genetics Graft Survival Kruppel-Lik;"
Notes:		"MedlineAi, Zongyong Xiang, Zheng Li, Yuemin Liu, Guoku Wang, Hong Zheng, Yun Qiu, Xiaoyan Zhao, Shumei Zhu, Xiaoqing Li, Yanhua Ji, Weizhi Li, Tianqing eng Comparative Study Research Support, Non-U.S. Gov't Netherlands 2015/11/20 Biomaterials. 2016 Jan; 77:53-65. doi: 10.1016/j.biomaterials.2015.10.079. Epub 2015 Oct 31"