| Title: | Genomics assisted functional characterization of Paenibacillus polymyxa HK4 as a biocontrol and plant growth promoting bacterium |
| Author(s): | Soni R; Rawal K; Keharia H; |
| Address: | "Department of Biosciences, UGC-Centre of Advanced Study, Sardar Patel University, Satellite Campus, Vadtal Road, Bakrol, 388 315, Anand, Gujarat, India. Department of Biosciences, UGC-Centre of Advanced Study, Sardar Patel University, Satellite Campus, Vadtal Road, Bakrol, 388 315, Anand, Gujarat, India. Electronic address: haresh970@gmail.com" |
| DOI: | 10.1016/j.micres.2021.126734 |
| ISSN/ISBN: | 1618-0623 (Electronic) 0944-5013 (Linking) |
| Abstract: | "The diseases caused by phytopathogens account for huge economic losses in the agricultural sector. Paenibacillus polymyxa is one of the agriculturally important biocontrol agents and plant growth promoting bacterium. This study describes the antifungal potential of P. polymyxa HK4 against an array of fungal phytopathogens and its ability to stimulate seed germination of cumin and groundnut under in vitro conditions. The cumin and groundnut seeds bacterized with HK4 exhibited enhanced germination efficiency in comparison to controls. The use of HK4 as a soil inoculant significantly promoted the shoot length and fresh weight of groundnut plants in pot studies. The draft genome analysis of HK4 revealed the genetic attributes for motility, root colonization, antagonism, phosphate solubilization, siderophore production and production of volatile organic compounds. The bacterium HK4 harnessed several hydrolytic enzymes that may assist its competence in the rhizosphere. The PCR amplification and sequence analysis of the conserved region of the fusA gene amplicon revealed the ability of HK4 to produce fusaricidin. Furthermore, the LC-ESI-MS/MS of crude cell pellet extract of HK4 confirmed the presence of fusaricidin as a major antifungal metabolite. This study demonstrated the potential of HK4 as a biocontrol agent and a plant growth promoter" |
| Keywords: | Antifungal Agents/chemistry/metabolism/pharmacology Bacterial Proteins/analysis/metabolism/pharmacology Crop Protection/*methods Cuminum/growth & development/*microbiology Depsipeptides/analysis/metabolism/pharmacology Fungi/drug effects/physiology Genome; |
| Notes: | "MedlineSoni, Riteshri Rawal, Khushboo Keharia, Hareshkumar eng Evaluation Study Germany 2021/03/11 Microbiol Res. 2021 Jul; 248:126734. doi: 10.1016/j.micres.2021.126734. Epub 2021 Mar 1" |
