| Title: | High infestation levels of Schizotetranychus oryzae severely affects rice metabolism |
| Author(s): | Blasi EAR; Buffon G; Rativa AGS; Lopes MCB; Berger M; Santi L; Lavallee-Adam M; Yates JR; Schwambach J; Beys-da-Silva WO; Sperotto RA; |
| Address: | "Programa de Pos-Graduacao em Biotecnologia (PPGBiotec), University of Taquari Valley - UNIVATES, Lajeado, RS, Brazil. Centro de Ciencias Biologicas e da Saude (CCBS), University of Taquari Valley - UNIVATES, Lajeado, RS, Brazil. Setor de Melhoramento Genetico, Instituto Rio Grandense do Arroz (IRGA), Cachoeirinha, RS, Brazil. Centro de Pesquisa Experimental, Hospital de Clinicas de Porto Alegre (CPE - HCPA/UFRGS), Porto Alegre, RS, Brazil. Ottawa Institute of Systems Biology and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada; Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA. Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA. Programa de Pos-Graduacao em Biotecnologia (PPGBiotec), University of Caxias do Sul (UCS), Caxias do Sul, RS, Brazil. Programa de Pos-Graduacao em Biotecnologia (PPGBiotec), University of Taquari Valley - UNIVATES, Lajeado, RS, Brazil; Centro de Ciencias Biologicas e da Saude (CCBS), University of Taquari Valley - UNIVATES, Lajeado, RS, Brazil. Electronic address: rasperotto@univates.br" |
| DOI: | 10.1016/j.jplph.2017.10.005 |
| ISSN/ISBN: | 1618-1328 (Electronic) 0176-1617 (Linking) |
| Abstract: | "High levels of Schizotetranychus oryzae phytophagous mite infestation on rice leaves can severely affect productivity. Physiological characterization showed that S. oryzae promotes a decrease in chlorophyll concentration and the establishment of a senescence process in rice leaves. Late-infested leaves also present high levels of superoxide radical and hydrogen peroxide accumulation, along with high levels of membrane integrity loss, which is indicative of cell death. To better understand the rice molecular responses to high levels of mite infestation, we employed the Multidimensional Protein Identification Technology (MudPIT) approach to identify differentially expressed proteins. We identified 83 and 88 proteins uniquely present in control and late-infested leaves, respectively, along with 11 and one proteins more abundant in control and late-infested leaves, respectively. S. oryzae infestation induces a decreased abundance of proteins related to translation, protease inhibition, and photosynthesis. On the other hand, infestation caused increased abundance of proteins involved in protein modification and degradation. Our results also suggest that S. oryzae infestation interferes with intracellular transport, DNA structure maintenance, and amino acid and lipid metabolism in rice leaves. Proteomic data were positively correlated with enzymatic assays and RT-qPCR analysis. Our findings describe the protein expression patterns of late-infested rice leaves and suggest several targets which could be tested in future biotechnological approaches aiming to avoid the population increase of phytophagous mite in rice plants" |
| Keywords: | Animals Food Chain Herbivory Oryza/*metabolism Photosynthesis Plant Leaves/metabolism Plant Proteins/*metabolism *Proteome Tetranychidae/*physiology MudPIT Oxidative stress Protease inhibitor Rice infestation Senescence Shotgun proteomics Translation; |
| Notes: | "MedlineBlasi, Edina A R Buffon, Giseli Rativa, Angie G S Lopes, Mara C B Berger, Markus Santi, Lucelia Lavallee-Adam, Mathieu Yates, John R 3rd Schwambach, Joseli Beys-da-Silva, Walter O Sperotto, Raul A eng Germany 2017/11/03 J Plant Physiol. 2017 Dec; 219:100-111. doi: 10.1016/j.jplph.2017.10.005. Epub 2017 Oct 28" |
