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Food Addit Contam Part A Chem Anal Control Expo Risk Assess

Title:		"Chemical analysis, antifungal and antimycotoxigenic activity of tetradenia riparia essential oil and crude extract"
Author(s):		Scanavacca J; Iecher Faria MG; Canonico Silva GC; Inumaro RS; Goncalves JE; Kupski L; Gazim ZC;
Address:		"Laboratorio de Quimica de Produtos Naturais, Programa de Pos graduacao de Biotecnologia Aplicada a Agricultura, Universidade Paranaense, Umuarama Parana, Brasil. Laboratorio de Microbiologia, Programa de Pos graduacao de Biotecnologia Aplicada a Agricultura, Universidade Paranaense, Umuarama Parana, Brasil. Laboratorio de Quimica, Programa de Pos Graduacao em tecnologias Limpas-PPGTL, Universidade Cesumar-UNICESUMAR, Campus Maringa, Parana Brasil. Instituto de Ciencia, Tecnologia e Inovacao ICETI UniCesumar, Maringa, Brasil. Laboratorio de Microbiologia, Departamento de Tecnologia, Universidade Estadual de Maringa, Umuarama, Brasil"
Journal Title:		Food Addit Contam Part A Chem Anal Control Expo Risk Assess
Year:		2022
Volume:		20220602
Issue:		7
Page Number:		1296 - 1310
DOI:		10.1080/19440049.2022.2080870
ISSN/ISBN:		1944-0057 (Electronic) 1944-0057 (Linking)
Abstract:		"Tetradenia riparia is known for its richness in essential oil which has been widely investigated due to its biological activities such as antimicrobial, insecticidal, trypanocidal, antimalarial and antioxidant. The objective of this work was to chemically analyze and evaluate the antifungal and antimycotoxigenic activity of the essential oil and the crude extract of leaves, flower buds and stems of T. riparia from the northwest region of the state of Parana. The essential oil was obtained by hydrodistillation using a Clevenger-type apparatus. To obtain the crude extract, the leaves, flower buds and stems were pulverized and subjected to a dynamic maceration process using 70% v v(-1) ethyl alcohol. Chemical analysis of the essential oil was performed by GC/MS, and chemical identification of the crude extract by UHPLC-ESI/qTOF. Antifungal activity (Rhizopus oryzae, Aspergillus flavus, Aspergillus ochraceus, Penicillium verrucosum and Fusarium graminearum) was performed by broth microdilution and the antimycotoxigenic assay was performed with A. ochraceus and P. verrucosum. Ochratoxin A was extracted by partition with chloroform and quantified by HPLC-FL. The oil yield was 0.29% for leaves, 0.34% for stems and 0.38% for flower buds, and the major compounds were fenchone, beta-caryophyllene, alpha-cadinol, 14-hydroxy-9- epi-caryophyllene, 9beta,13beta-epoxy-7-abietene, alpha-cadinol and 6-7-dehydroroyleanone. The main chemical compounds identified in the crude extract were terpenes, anthocyanins, flavonoids, tannins and phenolic acids. The minimum inhibitory concentration (MIC) of oils from leaves, flower buds and stems for the strains tested ranged from 0.87 mg mL(-1) to 33.3 mg mL(-1), while the minimum fungicidal concentration (MFC) ranged from 6.94 mg mL(-1) and 33.3 mg mL(-1). The MIC and MFC for ketoconazole, tebuconazole, sorbate and nitrite ranged from 0.05 to 33.3 mg mL(-1). The oil and crude extract of leaves, stems and flower buds showed an inhibition of ochratoxin A production for P. verrucosum of approximately 100%"
Keywords:		"Anthocyanins/analysis Antifungal Agents/analysis/pharmacology *Lamiaceae/chemistry Microbial Sensitivity Tests *Oils, Volatile/chemistry Plant Extracts/chemistry Plant Leaves/chemistry Fungicide floral buds leaves ochratoxin A stems;"
Notes:		"MedlineScanavacca, Juliana Iecher Faria, Maria Graciela Canonico Silva, Gabriela Catuzo Inumaro, Rodrigo Sadao Goncalves, Jose Eduardo Kupski, Larine Gazim, Zilda Cristiani eng England 2022/06/03 Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Jul; 39(7):1296-1310. doi: 10.1080/19440049.2022.2080870. Epub 2022 Jun 2"