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Mol Cell Biol

Title:		A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the a1-alpha 2 repressor
Author(s):		Sadhu C; Hoekstra D; McEachern MJ; Reed SI; Hicks JB;
Address:		"Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037"
Journal Title:		Mol Cell Biol
Year:		1992
Volume:		12
Issue:		5
Page Number:		1977 - 1985
DOI:		10.1128/mcb.12.5.1977-1985.1992
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"We have isolated a gene, designated CAG1, from Candida albicans by using the G-protein alpha-subunit clone SCG1 of Saccharomyces cerevisiae as a probe. Amino acid sequence comparison revealed that CAG1 is more homologous to SCG1 than to any other G protein reported so far. Homology between CAG1 and SCG1 not only includes the conserved guanine nucleotide binding domains but also spans the normally variable regions which are thought to be involved in interaction with the components of the specific signal transduction pathway. Furthermore, CAG1 contains a central domain, previously found only in SCG1. cag1 null mutants of C. albicans created by gene disruption produced no readily detectable phenotype. The C. albicans CAG1 gene complemented both the growth and mating defects of S. cerevisiae scg1 null mutants when carried on either a low- or high-copy-number plasmid. In diploid C. albicans, the CAG1 transcript was readily detectable in mycelial and yeast cells of both the white and opaque forms. However, the CAG1-specific transcript in S. cerevisiae transformants containing the C. albicans CAG1 gene was observed only in haploid cells. This transcription pattern matches that of SCG1 in S. cerevisiae and is caused by a1-alpha 2 mediated repression in diploid cells. That is, CAG1 behaves as a haploid-specific gene in S. cerevisiae, subject to control by the a1-alpha 2 mating-type regulation pathway. We infer from these results that C. albicans may have a signal transduction system analogous to that controlling mating type in S. cerevisiae or possibly even a sexual pathway that has so far remained undetected"
Keywords:		"Amino Acid Sequence Base Sequence Binding Sites Candida albicans/*genetics/physiology Crosses, Genetic Fungal Proteins/*metabolism GTP-Binding Proteins/*genetics/*metabolism *Genes, Fungal Haploidy *Homeodomain Proteins Macromolecular Substances Molecular;"
Notes:		"MedlineSadhu, C Hoekstra, D McEachern, M J Reed, S I Hicks, J B eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1992/05/01 Mol Cell Biol. 1992 May; 12(5):1977-85. doi: 10.1128/mcb.12.5.1977-1985.1992"