« Previous AbstractIon mobility spectrometry for pharmacokinetic studies--exemplary application    Next AbstractBreath profiles of children on ketogenic therapy »

J Chromatogr A

Title:		Multi-capillary-column proton-transfer-reaction time-of-flight mass spectrometry
Author(s):		Ruzsanyi V; Fischer L; Herbig J; Ager C; Amann A;
Address:		"Department of Anesthesiology and Critical Care Medicine, Innsbruck Medical University, Anichstrasse 35, A-6020 Innsbruck, Austria; Breath Research Institute, Austrian Academy of Sciences, Austria. Electronic address: veronika.ruzsanyi@i-med.ac.at"
Journal Title:		J Chromatogr A
Year:		2013
Volume:		20130925
Issue:
Page Number:		112 - 118
DOI:		10.1016/j.chroma.2013.09.072
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Print) 0021-9673 (Linking)
Abstract:		"Proton-transfer-reaction time-of-flight mass-spectrometry (PTR-TOFMS) exhibits high selectivity with a resolution of around 5000 m/Deltam. While isobars can be separated with this resolution, discrimination of isomeric compounds is usually not possible. The coupling of a multi-capillary column (MCC) with a PTR-TOFMS overcomes these problems as demonstrated in this paper for the ketone isomers 3-heptanone and 2-methyl-3-hexanone and for different aldehydes. Moreover, fragmentation of compounds can be studied in detail which might even improve the identification. LODs for compounds tested are in the range of low ppbv and peak positions of the respective separated substances show good repeatability (RSD of the peak positions <3.2%). Due to its special characteristics, such as isothermal operation, compact size, the MCC setup is suitable to be installed inside the instrument and the overall retention time for a complete spectrum is only a few minutes: this allows near real-time measurements in the optional MCC mode. In contrast to other methods that yield additional separation, such as the use of pre-cursor ions other than H3O(+), this method yields additional information without increasing complexity"
Keywords:		Breath Tests Gas Chromatography-Mass Spectrometry/*instrumentation/methods Humans Ions Protons Skin/chemistry/metabolism Volatile Organic Compounds/*analysis/isolation & purification Gas chromatographic separation Multi capillary column Ptr-tofms Proton t;
Notes:		"MedlineRuzsanyi, Veronika Fischer, Lukas Herbig, Jens Ager, Clemes Amann, Anton eng Research Support, Non-U.S. Gov't Netherlands 2013/10/15 J Chromatogr A. 2013 Nov 5; 1316:112-8. doi: 10.1016/j.chroma.2013.09.072. Epub 2013 Sep 25"