| Title: | High-Throughput Breath Volatile Organic Compound Analysis Using Thermal Desorption Proton Transfer Reaction Time-of-Flight Mass Spectrometry |
| Author(s): | Romano A; Doran S; Belluomo I; Hanna GB; |
| Address: | "Department of Surgery and Cancer , Imperial College London , 10th Floor QEQM Building, St. Mary's Hospital, South Wharf Road , London W2?ª+1NY , United Kingdom" |
| DOI: | 10.1021/acs.analchem.8b01045 |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "Breath analysis is highly acceptable to patients and health care professionals, but its implementation in clinical practice remains challenging. Clinical trials and routine practice require a robust system for collection, storage, and processing of large numbers of samples. This work describes a platform based upon the hyphenation of thermal desorption (TD) with proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS), coupled by means of an original modification of the TD interface. The performance of TD-PTR-ToF-MS was tested against seven oxygenated volatile organic compounds (VOCs), belonging to three chemical classes (i.e., fatty acids, aldehydes, and phenols), previously identified as possible biomarkers of colorectal and esophago-gastric adenocarcinoma. Limits of detection and quantification were on the order of 0.2-0.9 and 0.3-1.5 parts per billion by volume (ppbV), respectively. Analytical recoveries from TD tubes were 80% or higher, linear response was in the low- to mid-ppbV range ( R(2) = 0.98-0.99), and coefficients of variation were within 20% of mean values. The usability of the platform was evaluated in the analysis of a set of breath samples of clinical origin, allowing for a throughput of nearly 100 TD tubes for 24 h of continuous operation. All of these characteristics enhance the implementation of TD-PTR-ToF-MS for large-scale clinical studies" |
| Keywords: | Aldehydes/analysis *Breath Tests Humans Mass Spectrometry/*methods Protons Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineRomano, Andrea Doran, Sophie Belluomo, Ilaria Hanna, George Bushra eng MC_PC_15028/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't 2018/08/15 Anal Chem. 2018 Sep 4; 90(17):10204-10210. doi: 10.1021/acs.analchem.8b01045. Epub 2018 Aug 14" |
