| Title: | New Insights on Endophytic Microbacterium-Assisted Blast Disease Suppression and Growth Promotion in Rice: Revelation by Polyphasic Functional Characterization and Transcriptomics |
| Author(s): | Patel A; Sahu KP; Mehta S; Javed M; Balamurugan A; Ashajyothi M; Sheoran N; Ganesan P; Kundu A; Gopalakrishnan S; Gogoi R; Kumar A; |
| Address: | "Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India" |
| DOI: | 10.3390/microorganisms11020362 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Plant growth-promoting endophytic microbes have drawn the attention of researchers owing to their ability to confer fitness benefits in many plant species. Here, we report agriculturally beneficial traits of rice-leaf-adapted endophytic Microbacterium testaceum. Our polyphasic taxonomic investigations revealed its identity as M. testaceum. The bacterium displayed typical endophytism in rice leaves, indicated by the green fluorescence of GFP-tagged M. testaceum in confocal laser scanning microscopy. Furthermore, the bacterium showed mineral solubilization and production of IAA, ammonia, and hydrolytic enzymes. Tobacco leaf infiltration assay confirmed its non-pathogenic nature on plants. The bacterium showed antifungal activity on Magnaporthe oryzae, as exemplified by secreted and volatile organic metabolome-mediated mycelial growth inhibition. GC-MS analysis of the volatilome of M. testaceum indicated the abundance of antimicrobial compounds. Bacterization of rice seedlings showed phenotypic traits of MAMP-triggered immunity (MTI), over-expression of OsNPR1 and OsCERK, and the consequent blast suppressive activity. Strikingly, M. testaceum induced the transcriptional tradeoff between physiological growth and host defense pathways as indicated by up- and downregulated DEGs. Coupled with its plant probiotic features and the defense elicitation activity, the present study paves the way for developing Microbacterium testaceum-mediated bioformulation for sustainably managing rice blast disease" |
| Keywords: | DEGs Magnaporthe Microbacterium testaceum blast disease probiotic features transcriptome assembly; |
| Notes: | "PubMed-not-MEDLINEPatel, Asharani Sahu, Kuleshwar Prasad Mehta, Sahil Javed, Mohammed Balamurugan, Alexander Ashajyothi, Mushineni Sheoran, Neelam Ganesan, Prakash Kundu, Aditi Gopalakrishnan, Subbaiyan Gogoi, Robin Kumar, Aundy eng BT/BIRAC/SITARE-GYTI363/DBT-SITARE GYTI 2021/ Switzerland 2023/02/26 Microorganisms. 2023 Jan 31; 11(2):362. doi: 10.3390/microorganisms11020362" |
