| Title: | "'Alternative self-diploidization' or 'ASD' homothallism in Saccharomyces cerevisiae: isolation of a mutant, nuclear-cytoplasmic interaction and endomitotic diploidization" |
| Author(s): | Ono B; Ishino-Arao Y; Takasugi K; Taniguchi M; Fukuda M; Fukui M; Miyakawa I; Sando N; |
| Address: | "Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan" |
| DOI: | 10.1093/genetics/125.4.729 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "A mutant of Saccharomyces cerevisiae representing a novel life cycle, named 'alternative self-diploidization' or 'ASD' homothallism, was obtained fortuitously. In this life cycle, MAT alpha (or MATa) haplophase and MAT alpha/MAT alpha (or MATa/MATa) diplophase alternate. Germinated cells are haploid and mating. They soon become nonmating and sporogenous as they vegetatively grow. They sooner or later diploidize presumably via endomitosis. The diploid cells haploidize via normal meiosis. A single recessive nuclear mutation, named asd 1-1, is responsible for 'ASD' homothallism. In the rho 0 cytoplasm, asd 1-1 cells mate even if at a low efficiency and fail to diploidize. Since pet mutations do not have such effects, we conclude that a certain mitochondrial function other than respiration is required for manifestation of 'ASD' homothallism. That is, 'ASD' homothallism is the result of some sort of nuclear-cytoplasmic interaction" |
| Keywords: | "Cell Nucleus/*metabolism Cloning, Molecular Cytoplasm/*metabolism *Diploidy Genes, Fungal Genes, Recessive Mating Factor *Mutation Peptides/pharmacology Polyploidy Saccharomyces cerevisiae/*genetics/growth & development/physiology Spores, Fungal;" |
| Notes: | "MedlineOno, B Ishino-Arao, Y Takasugi, K Taniguchi, M Fukuda, M Fukui, M Miyakawa, I Sando, N eng Research Support, Non-U.S. Gov't 1990/08/01 Genetics. 1990 Aug; 125(4):729-38. doi: 10.1093/genetics/125.4.729" |
