| Title: | Cross-species microarray hybridization to identify developmentally regulated genes in the filamentous fungus Sordaria macrospora |
| Author(s): | Nowrousian M; Ringelberg C; Dunlap JC; Loros JJ; Kuck U; |
| Address: | "Lehrstuhl fur Allgemeine und Molekulare Botanik, Ruhr-Universitat Bochum ND 7/131, Universitatsstr. 150, 44801, Bochum, Germany" |
| DOI: | 10.1007/s00438-005-1118-9 |
| ISSN/ISBN: | 1617-4615 (Print) 1617-4623 (Linking) |
| Abstract: | "The filamentous fungus Sordaria macrospora forms complex three-dimensional fruiting bodies that protect the developing ascospores and ensure their proper discharge. Several regulatory genes essential for fruiting body development were previously isolated by complementation of the sterile mutants pro1, pro11 and pro22. To establish the genetic relationships between these genes and to identify downstream targets, we have conducted cross-species microarray hybridizations using cDNA arrays derived from the closely related fungus Neurospora crassa and RNA probes prepared from wild-type S. macrospora and the three developmental mutants. Of the 1,420 genes which gave a signal with the probes from all the strains used, 172 (12%) were regulated differently in at least one of the three mutants compared to the wild type, and 17 (1.2%) were regulated differently in all three mutant strains. Microarray data were verified by Northern analysis or quantitative real time PCR. Among the genes that are up- or down-regulated in the mutant strains are genes encoding the pheromone precursors, enzymes involved in melanin biosynthesis and a lectin-like protein. Analysis of gene expression in double mutants revealed a complex network of interaction between the pro gene products" |
| Keywords: | "Blotting, Northern *Gene Expression Regulation, Fungal Genes, Regulator/*genetics *Hybridization, Genetic Mutation/genetics Neurospora crassa/genetics Oligonucleotide Array Sequence Analysis Oligonucleotides Sordariales/*genetics Spores, Fungal/*genetics;" |
| Notes: | "MedlineNowrousian, Minou Ringelberg, Carol Dunlap, Jay C Loros, Jennifer J Kuck, Ulrich eng P01 GM068087/GM/NIGMS NIH HHS/ R01 GM034985/GM/NIGMS NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Germany 2005/03/22 Mol Genet Genomics. 2005 Apr; 273(2):137-49. doi: 10.1007/s00438-005-1118-9. Epub 2005 Mar 19" |
