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Methods Mol Biol

Title:		Analysis of volatile mouse pheromones by gas chromatography mass spectrometry
Author(s):		Novotny MV; Soini HA;
Address:		"Department of Chemistry, Institute for Pheromone Research, Indiana University, Bloomington, IN, USA"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		29 - 45
DOI:		10.1007/978-1-62703-619-1_3
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"High-precision quantitative profiling of volatile organic constituents in rodent physiological fluids and glandular secretions is needed to relate olfactory signals to physiology and behavior. Whereas capillary gas chromatography-mass spectrometry (GC-MS) analysis has become the most widely applied in such investigations, the extraction and preconcentration of volatile organics is arguably the most critical step in the overall analytical task. In this chapter, we describe technical details of two main sample extraction procedures used in our laboratory: dynamic headspace trapping, and stir bar sorptive extraction (SBSE). They have been demonstrated here for the chromatographic analysis of mouse urine, serum, saliva, and preputial gland specimens"
Keywords:		Animals Body Fluids/*chemistry Exocrine Glands/chemistry Gas Chromatography-Mass Spectrometry/*methods Mice Pheromones/*analysis/chemistry Saliva/chemistry Serum/chemistry Urine/chemistry Volatile Organic Compounds/*analysis/*chemistry;
Notes:		"MedlineNovotny, Milos V Soini, Helena A eng Research Support, Non-U.S. Gov't 2013/09/10 Methods Mol Biol. 2013; 1068:29-45. doi: 10.1007/978-1-62703-619-1_3"