Title: | "Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1" |
Author(s): | Nakayama J; Nagasawa H; Isogai A; Clewell DB; Suzuki A; |
Address: | "Department of Agricultural Chemistry, University of Tokyo, Japan" |
DOI: | 10.1016/0014-5793(90)81019-k |
ISSN/ISBN: | 0014-5793 (Print) 0014-5793 (Linking) |
Abstract: | "The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-BglII 3.6-kbp fragment of the plasmid pAM351(pPdl::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gln-Gln-Pro repeat in the C-terminal region of TraD product encoded on the R100 plasmid in Escherichia coli" |
Keywords: | "Amino Acid Sequence *Bacterial Adhesion Bacterial Proteins/*genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Enterococcus faecalis/*genetics *Genes, Bacterial Molecular Sequence Data Pheromones *Plasmids;" |
Notes: | "MedlineNakayama, J Nagasawa, H Isogai, A Clewell, D B Suzuki, A eng GM33956/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1990/07/30 FEBS Lett. 1990 Jul 30; 268(1):245-8. doi: 10.1016/0014-5793(90)81019-k" |