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Methods Mol Biol

Title:		Functional assays for insect olfactory receptors in Xenopus oocytes
Author(s):		Nakagawa T; Touhara K;
Address:		"Biological/Pharmacological Research Laboratories, Japan Tobacco Inc., Central Pharmaceutical Research Institute, Osaka, Japan"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		107 - 119
DOI:		10.1007/978-1-62703-619-1_8
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Reconstitution of olfactory or pheromone receptors in heterologous expression systems greatly facilitates the functional analysis of these receptors. Xenopus laevis oocytes can be used to efficiently express insect olfactory or pheromone receptors. In this chapter, we describe how to use Xenopus laevis oocytes for functional assays of insect olfactory receptors. The procedure can be subdivided into the four following steps: (1) in vitro complementary RNA (cRNA) synthesis, (2) isolation of oocytes from female Xenopus laevis, (3) cRNA microinjection into oocytes, and (4) two-electrode voltage-clamp recording. This system can be used to identify odor or pheromone ligands and to analyze structure-function relationships involving receptor proteins of interest"
Keywords:		"Animals Drosophila/metabolism Drosophila Proteins/analysis/chemistry/*genetics Female Microinjections Oocytes/cytology/*metabolism Patch-Clamp Techniques RNA, Complementary/chemical synthesis Receptors, Odorant/analysis/chemistry/*genetics Receptors, Pher;"
Notes:		"MedlineNakagawa, Tatsuro Touhara, Kazushige eng 2013/09/10 Methods Mol Biol. 2013; 1068:107-19. doi: 10.1007/978-1-62703-619-1_8"