« Previous AbstractAgnihotra Yajna: A Prototype of South Asian Traditional Medical Knowledge    Next AbstractNatural dietary anti-cancer chemopreventive compounds: redox-mediated differential signaling mechanisms in cytoprotection of normal cells versus cytotoxicity in tumor cells »

Elife

Title:		Multiplexed mRNA assembly into ribonucleoprotein particles plays an operon-like role in the control of yeast cell physiology
Author(s):		Nair RR; Zabezhinsky D; Gelin-Licht R; Haas BJ; Dyhr MC; Sperber HS; Nusbaum C; Gerst JE;
Address:		"Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel. Broad Institute of MIT and Harvard, Cambridge, United States"
Journal Title:		Elife
Year:		2021
Volume:		20210504
Issue:
Page Number:		-
DOI:		10.7554/eLife.66050
ISSN/ISBN:		2050-084X (Electronic) 2050-084X (Linking)
Abstract:		"Prokaryotes utilize polycistronic messages (operons) to co-translate proteins involved in the same biological processes. Whether eukaryotes achieve similar regulation by selectively assembling and translating monocistronic messages derived from different chromosomes is unknown. We employed transcript-specific RNA pulldowns and RNA-seq/RT-PCR to identify yeast mRNAs that co-precipitate as ribonucleoprotein (RNP) complexes. Consistent with the hypothesis of eukaryotic RNA operons, mRNAs encoding components of the mating pathway, heat shock proteins, and mitochondrial outer membrane proteins multiplex in trans, forming discrete messenger ribonucleoprotein (mRNP) complexes (called transperons). Chromatin capture and allele tagging experiments reveal that genes encoding multiplexed mRNAs physically interact; thus, RNA assembly may result from co-regulated gene expression. Transperon assembly and function depends upon histone H4, and its depletion leads to defects in RNA multiplexing, decreased pheromone responsiveness and mating, and increased heat shock sensitivity. We propose that intergenic associations and non-canonical histone H4 functions contribute to transperon formation in eukaryotic cells and regulate cell physiology"
Keywords:		"Gene Expression Histones/genetics/metabolism *Operon RNA, Messenger/genetics/*metabolism Ribonucleoproteins/genetics/*metabolism Saccharomyces cerevisiae/*genetics/*physiology RNA-binding proteins S.cerevisiae cell biology heat shock proteins histone H4;"
Notes:		"MedlineNair, Rohini R Zabezhinsky, Dmitry Gelin-Licht, Rita Haas, Brian J Dyhr, Michael Ca Sperber, Hannah S Nusbaum, Chad Gerst, Jeffrey E eng Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2021/05/05 Elife. 2021 May 4; 10:e66050. doi: 10.7554/eLife.66050"