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Biosens Bioelectron

Title:		"Chopper-stabilized gas chromatography-electroantennography: Part I. background, signal processing and example"
Author(s):		Myrick AJ; Baker TC;
Address:		"Dept. of Entomology, Center for Chemical Ecology, 104 Chemical Ecology Laboratory, Penn State University, University Park, PA 16802, USA. ajm25@psu.edu"
Journal Title:		Biosens Bioelectron
Year:		2012
Volume:		20111019
Issue:		1
Page Number:		197 - 204
DOI:		10.1016/j.bios.2011.10.017
ISSN/ISBN:		1873-4235 (Electronic) 0956-5663 (Linking)
Abstract:		"A new method that can improve gas-chromatography-electroantennographic detection (GC-EAD) by orders of magnitude through a technique known as chopper stabilization combined with matched filtering in colored noise is presented. The EAD is a physiological recording from the antenna of an insect which can be used to find compounds in the GC effluent that the antenna is able to detect, having important applications for pest control and understanding of chemical communication in nature. The new method is demonstrated with whole-animal male Helicoverpa zea antennal preparations for detection of major pheromone component (cis-11-hexadecenal) and compared to results obtained using traditional EAD recording techniques. Results indicate that chopper stabilization under these circumstances can increase odorant detection performance by a factor of approximately 10(4) over traditional methods. The time course of the response of the antenna is also better resolved under chopped conditions. Although the degree of improvement is expected to vary with insect species, odor, and insect preparation, under most circumstances a more sensitive and robust GC-EAD instrument will result from the application of this technique"
Keywords:		"Animals Biological Assay/*instrumentation/methods Biosensing Techniques/*instrumentation Chromatography, Gas/*instrumentation/methods Equipment Design Equipment Failure Analysis Lepidoptera/*chemistry/drug effects/physiology Male Odorants/*analysis Reprod;"
Notes:		"MedlineMyrick, Andrew J Baker, Thomas C eng Research Support, U.S. Gov't, Non-P.H.S. England 2011/11/25 Biosens Bioelectron. 2012 Jan 15; 31(1):197-204. doi: 10.1016/j.bios.2011.10.017. Epub 2011 Oct 19"