| Title: | Evaluation of pheromone-based kit: A noninvasive approach of estrus detection in buffalo |
| Author(s): | Muthukumar S; Muniasamy S; Srinivasan M; Ilangovan A; Satheshkumar S; Rajagopal T; Ramesh Saravana Kumar V; Sivakumar K; Archunan G; |
| Address: | "Center for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India. Center for Animal Research, Training and Services (CAReTS), Central Inter-Disciplinary Research Facility (CIDRF), Mahatma Gandhi Medical College and Research Institute (MGMC-RI) Campus, Puducherry, India. Department of Microbiology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Tamil Nadu, India. School of Chemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India. Department of Animal Biotechnology, Madras Veterinary College, Chennai, Tamil Nadu, India. Post Graduate and Research Department of Zoology and Microbiology, Thiagarajar College (Autonomous), Madurai, Tamil Nadu, India. Department of Livestock Production and Management, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India" |
| ISSN/ISBN: | 1439-0531 (Electronic) 0936-6768 (Linking) |
| Abstract: | "In view of the silent nature of estrus in buffalo, a noninvasive assay kit has long been felt necessary for easy and effective estrus detection. This study was designed to detect estrus in buffalo using a kit formulated in our laboratory based on pheromone compound. Group I: Urine samples collected at estrus phase and group II: randomly collected urine samples were subjected to the test using the kit. No colour developed (i.e., positive reaction) in estrus urine after adding the kit solution. By contrast, pale and/or dark pink colour developed (i.e., negative reaction) in urine from the proestrus and diestrus buffaloes, respectively. Field evaluation of the kit in groups I and II revealed that 60.87% and 71.43% of urine samples were correctly identified as estrus and nonestrus (i.e., proestrus and diestrus), respectively. Therefore, the first of its kind estrus detection kit formulated based on urinary pheromone can as well be used as a simple device to detect estrus in buffalo" |
| Keywords: | Animals Buffaloes/physiology/*urine Cresols/*urine Estrus/urine Estrus Detection/*methods Female India Pheromones/*urine artificial insemination assay buffalo estrus cycle estrus detection kit pheromones reproduction urine; |
| Notes: | "MedlineMuthukumar, Subramanian Muniasamy, Samuthirapandi Srinivasan, Mahalingam Ilangovan, Andivelu Satheshkumar, Soundarapandian Rajagopal, Thangavel Ramesh Saravana Kumar, Velusamy Sivakumar, Karuppusamy Archunan, Govindaraju eng BT/PR/10247/AAQ/1/364/2007/Department of Biotechnology (DBT)/ Ministry of Science and Technology, New Delhi, India/ Germany 2018/07/26 Reprod Domest Anim. 2018 Dec; 53(6):1466-1472. doi: 10.1111/rda.13281. Epub 2018 Aug 22" |
