« Previous Abstract"Functional characterization of the three mitogen-activated protein kinase kinases (MAP2Ks) present in the Cryphonectria parasitica genome reveals the necessity of Cpkk1 and Cpkk2, but not Cpkk3, for pathogenesis on chestnut (Castanea spp.)"    Next AbstractDisruption by conophthorin of the kairomonal response of sawyer beetles to bark beetle pheromones »

BMC Microbiol

Title:		Effects of different cultivation conditions on the production of beta-cyclocitral and beta-ionone in Microcystis aeruginosa
Author(s):		Moretto JAS; de Freitas PNN; de Almeida EC; Altarugio LM; da Silva SV; de Fatima Fiore M; Pinto E;
Address:		"Centre for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil. jessica.moretto@usp.br. Centre for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil. Luiz de Queiroz College of Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil. Faculty of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil. Centre for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP, Brazil. ernani@usp.br. Faculty of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil. ernani@usp.br. Food Research Center (FoRC - CEPID), University of Sao Paulo, Sao Paulo, SP, Brazil. ernani@usp.br"
Journal Title:		BMC Microbiol
Year:		2022
Volume:		20220324
Issue:		1
Page Number:		78 -
DOI:		10.1186/s12866-022-02473-6
ISSN/ISBN:		1471-2180 (Electronic) 1471-2180 (Linking)
Abstract:		"BACKGROUND: Cyanobacteria blooms have become a major environmental problem and concern because of secondary metabolites produced by cyanobacteria released into the water. Cyanobacteria produce volatile organic compounds (VOCs), such as the compounds beta-cyclocitral and beta-ionone, which comprise odors, off-flavors, defense compounds, as well as growth regulators. Therefore, the general objective of this work was to evaluate the VOCs produced by two strains of Microcystis aeruginosa, differing in their ability to produce microcystins (LTPNA 01-non-producing and LTPNA 08-toxin-producing). The analysis of VOC production was carried out in (1) normal culture conditions, (2) under different light intensities (LI), and (3) after the external application of beta-ionone in both cultures. RESULTS: The results showed that beta-cyclocitral and beta-ionone are produced in all growth phases of LTPNA 01 and LTPNA 08. Both strains were producers of beta-cyclocitral and beta-ionone in normal culture conditions. It was observed that the beta-cyclocitral concentration was higher than beta-ionone in all light intensities investigated in this study. Additionally, the strain LTPNA 01 produced more beta-cyclocitral than LTPNA 08 at almost all times and LIs analyzed. However, the strain LTPNA 08 produced more beta-ionone, mainly at the initial times. In addition, the experiment results with the external addition of beta-ionone in the cultures showed that the strain LTPNA 01 produced more beta-cyclocitral in control conditions than in treatment. Nonetheless, beta-ionone production was higher in treatment conditions in LTPNA 08, indicating that the addition of beta-ionone may favor the production of these compounds and inhibit the production of beta-cyclocitral. CONCLUSION: Our results showed that some abiotic factors, such as different light intensities and external application of beta-ionone, can be triggers that lead to the production of VOCs"
Keywords:		Aldehydes/metabolism *Cyanobacteria/metabolism Diterpenes *Microcystis Norisoprenoids/metabolism *Volatile Organic Compounds/analysis Cyanobacteria Gc-ms Spme beta-cyclocitral beta-ionone;
Notes:		"MedlineMoretto, Jessica Aparecida Silva de Freitas, Paloma Nathane Nunes de Almeida, Eryka Costa Altarugio, Lucas Miguel da Silva, Simone Vieira de Fatima Fiore, Marli Pinto, Ernani eng Research Support, Non-U.S. Gov't England 2022/03/25 BMC Microbiol. 2022 Mar 24; 22(1):78. doi: 10.1186/s12866-022-02473-6"