| Title: | "Mimicking insect communication: release and detection of pheromone, biosynthesized by an alcohol acetyl transferase immobilized in a microreactor" |
| Author(s): | Munoz L; Dimov N; Carot-Sans G; Bula WP; Guerrero A; Gardeniers HJ; |
| Address: | "Department of Biological Chemistry and Molecular Modeling, IQAC (CSIC), Barcelona, Spain" |
| DOI: | 10.1371/journal.pone.0047751 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Infochemical production, release and detection of (Z,E)-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth Spodoptera littoralis, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an 'artificial gland', i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding (Z,E)-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered" |
| Keywords: | *Animal Communication Animals Female Male Pheromones/*chemistry Sex Attractants/*chemistry Spodoptera/*physiology; |
| Notes: | "MedlineMunoz, Lourdes Dimov, Nikolay Carot-Sans, Gerard Bula, Wojciech P Guerrero, Angel Gardeniers, Han J G E eng Research Support, Non-U.S. Gov't 2012/11/17 PLoS One. 2012; 7(11):e47751. doi: 10.1371/journal.pone.0047751. Epub 2012 Nov 14" |
