| Title: | Development of a Dye-Based Device to Assess the Poultry Meat Spoilage. Part II: Array on Act |
| Author(s): | Magnaghi LR; Alberti G; Capone F; Zanoni C; Mannucci B; Quadrelli P; Biesuz R; |
| Address: | "Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy. Unita di Ricerca di Pavia, INSTM, Via G. Giusti 9, 50121 Firenze, Italy. Centro Grandi Strumenti, University of Pavia, Via Bassi 21, 27100 Pavia, Italy" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Print) 0021-8561 (Linking) |
| Abstract: | "This work presents a colorimetric dye-based array for naked-eye detection of chicken meat spoilage. The array is obtained by fixing five acid-base indicators, m-cresol purple (1), o-cresol red (2), bromothymol blue (3), thymol blue (4), and chlorophenol red (5), and a sensing molecule specific for thiols, 5,5'-dithiobis(2-nitrodibenzoic acid), called Ellman's reagent (6), on a cellulose-based support. The dyes, being permanently charged, are fixed on the support via ion-exchange. The entire degradation process of beast poultry meat, at ambient temperature and in a domestic fridge, is followed by the change of the color of the array, placed in the headspace over the meat samples. The device is set after selection of the most suitable starting form, which could be the acidic or the basic color of indicators, being the proper dye concentration and the dimension of the spots already established. Basing on sensors colors, we identified three levels of the degradation process of chicken meat, named SAFE, WARNING, and HAZARD. By instrumental analysis, we demonstrated that sensors response was correlated to volatile organic compounds (VOCs) composition in the headspace and, thus, to meat spoilage progress. We demonstrated that biogenic amines (BAs), commonly considered a critical spoilage marker, are indeed produced into the samples but never present in the headspace, even in traces, during the investigated time-lapse. The VOC evolution nevertheless allows one to assign the sample as WARNING and further HAZARD. Some indicators turned out to be more informative than others, and the best candidates for a future industrial application resulted in a bromothymol blue (3)-, chlorophenol red (5)-, and Ellman's reagent (6)-based array" |
| Keywords: | Animals Bromthymol Blue/analysis Chickens Color Colorimetry Coloring Agents/*analysis Food Analysis/instrumentation/methods Food Safety Meat/*analysis Phenolsulfonphthalein/analogs & derivatives/analysis Thymolphthalein/analogs & derivatives/analysis Vola; |
| Notes: | "MedlineMagnaghi, Lisa Rita Alberti, Giancarla Capone, Federica Zanoni, Camilla Mannucci, Barbara Quadrelli, Paolo Biesuz, Raffaela eng 2020/10/30 J Agric Food Chem. 2020 Nov 11; 68(45):12710-12718. doi: 10.1021/acs.jafc.0c03771. Epub 2020 Oct 29" |
