| Title: | Identification and Functional Analysis of SlitOBP11 From Spodoptera litura |
| Author(s): | Luo J; Zhang Z; Li D; Liu J; Li K; Sun X; He L; |
| Address: | "Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China. Academy of Agricultural Sciences, Southwest University, Chongqing, China. State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, China. Laboratory of Forest Pathogen Integrated Biology, Research Institute of Forestry New Technology, Chinese Academy of Forestry, Beijing, China. State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China" |
| DOI: | 10.3389/fphys.2021.619816 |
| ISSN/ISBN: | 1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking) |
| Abstract: | "Odorant binding proteins (OBPs) play a key role in the olfactory recognition of insects, whose functions have been extensively studied in adult insects but rarely in larvae. In this study, one OBP (SlitOBP11) with high expression in larval antenna but low expression in adult antenna of Spodoptera litura was screened by RNA-seq and verified by quantitative real-time PCR. Furthermore, the function of SlitOBP11 was explored by analysis of the expression patterns and prokaryotic expression of proteins as well as assays of competitive binding. Competitive binding assay demonstrated that SlitOBP11 had high binding affinity to all four female sex pheromone components, but exhibited almost no binding affinity to plant volatiles except for a low affinity to Phenylacetaldehyde and Phenethyl acetate. Homology modeling and molecular docking implied that the shape of these four sex pheromones were linear, which were appropriate for the binding channel of SlitOBP11 and the amino acid residue Asn99 of SlitOBP11 might play an important role in binding. Taken together, our results indicate that SlitOBP11 may be involved in the perception of female sex pheromones by S. litura larvae, and OBPs in the larvae of S. litura play an important role in the olfactory perception process" |
| Keywords: | SlitOBP11 Spodoptera litura larvae olfactory odorant binding protein sex pheromone; |
| Notes: | "PubMed-not-MEDLINELuo, Jiaojiao Zhang, Zan Li, Dongzhen Liu, Jie Li, Kun Sun, Xiao He, Lin eng Switzerland 2021/03/02 Front Physiol. 2021 Feb 11; 12:619816. doi: 10.3389/fphys.2021.619816. eCollection 2021" |
