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Mol Plant

Title:		A Conserved Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation
Author(s):		Liu Z; Boachon B; Lugan R; Tavares R; Erhardt M; Mutterer J; Demais V; Pateyron S; Brunaud V; Ohnishi T; Pencik A; Achard P; Gong F; Hedden P; Werck-Reichhart D; Renault H;
Address:		"Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique (CNRS), University of Strasbourg, 67084 Strasbourg, France. Laboratoire de Biometrie et Biologie Evolutive, Universite Lyon 1, CNRS, 69622 Villeurbanne, France. Plateforme d'Imagerie In Vitro, IFR 37 de Neurosciences, 67084 Strasbourg, France. Transcriptomic Platform, Unite de Recherche en Genomique Vegetale (URGV), INRA, Universite d'Evry Val d'Essonne, CNRS, 91057 Evry, France. Bioinformatics for Predictive Genomics, URGV, INRA, Universite d'Evry Val d'Essonne, CNRS, 91057 Evry, France. Graduate School of Agriculture, Shizuoka University, Shizuoka, 422-8529 Japan. Laboratory of Growth Regulators & Department of Chemical Biology and Genetics, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University & Institute of Experimental Botany AS CR, 771 47 Olomouc, Czech Republic. Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique (CNRS), University of Strasbourg, 67084 Strasbourg, France; University of Strasbourg Institute for Advanced Study (USIAS), 67084 Strasbourg, France; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 79104 Freiburg, Germany. Electronic address: daniele.werck@ibmp-cnrs.unistra.fr. Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique (CNRS), University of Strasbourg, 67084 Strasbourg, France; University of Strasbourg Institute for Advanced Study (USIAS), 67084 Strasbourg, France; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 79104 Freiburg, Germany"
Journal Title:		Mol Plant
Year:		2015
Volume:		20150918
Issue:		12
Page Number:		1751 - 1765
DOI:		10.1016/j.molp.2015.09.002
ISSN/ISBN:		1752-9867 (Electronic) 1674-2052 (Linking)
Abstract:		"Global inspection of plant genomes identifies genes maintained in low copies across taxa and under strong purifying selection, which are likely to have essential functions. Based on this rationale, we investigated the function of the low-duplicated CYP715 cytochrome P450 gene family that appeared early in seed plants and evolved under strong negative selection. Arabidopsis CYP715A1 showed a restricted tissue-specific expression in the tapetum of flower buds and in the anther filaments upon anthesis. cyp715a1 insertion lines showed a strong defect in petal development, and transient alteration of pollen intine deposition. Comparative expression analysis revealed the downregulated expression of genes involved in pollen development, cell wall biogenesis, hormone homeostasis, and floral sesquiterpene biosynthesis, especially TPS21 and several key genes regulating floral development such as MYB21, MYB24, and MYC2. Accordingly, floral sesquiterpene emission was suppressed in the cyp715a1 mutants. Flower hormone profiling, in addition, indicated a modification of gibberellin homeostasis and a strong disturbance of the turnover of jasmonic acid derivatives. Petal growth was partially restored by the active gibberellin GA3 or the functional analog of jasmonoyl-isoleucine, coronatine. CYP715 appears to function as a key regulator of flower maturation, synchronizing petal expansion and volatile emission. It is thus expected to be an important determinant of flower-insect interaction"
Keywords:		Arabidopsis/classification/enzymology/genetics/*growth & development Arabidopsis Proteins/chemistry/genetics/*metabolism Conserved Sequence Cytochrome P-450 Enzyme System/chemistry/genetics/*metabolism Flowers/classification/*enzymology/genetics/growth &;
Notes:		"MedlineLiu, Zhenhua Boachon, Benoit Lugan, Raphael Tavares, Raquel Erhardt, Mathieu Mutterer, Jerome Demais, Valerie Pateyron, Stephanie Brunaud, Veronique Ohnishi, Toshiyuki Pencik, Ales Achard, Patrick Gong, Fan Hedden, Peter Werck-Reichhart, Daniele Renault, Hugues eng BB/E006922/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BBS/E/C/00004951/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2015/09/22 Mol Plant. 2015 Dec 7; 8(12):1751-65. doi: 10.1016/j.molp.2015.09.002. Epub 2015 Sep 18"