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Sichuan Da Xue Xue Bao Yi Xue Ban

Title:		[Bactericidal peptide targeted against penicillin/methicillin-resistant Staphylococcus aureus--an engineered multidomain protein machine]
Author(s):		Liao Y; Zhang J; Lu X; Wan L; Li S; Chen G; Gao Y; Qiu X;
Address:		"Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China"
Journal Title:		Sichuan Da Xue Xue Bao Yi Xue Ban
Year:		2003
Volume:		34
Issue:		4
Page Number:		605 - 609
DOI:
ISSN/ISBN:		1672-173X (Print) 1672-173X (Linking)
Abstract:		"OBJECTIVE: To construct a targeting bactericidal peptide machine by fusing two minidomains with different bioactivities and different protein origins. METHODS: Such fusion peptide was constructed by linking the gene of Staphylococcal AgrD pheromone with the gene of C-terminal (I626) of colicin Ia pore-forming region (K544-I626) with site-directed mutation. Mutated plasmid was transformed into E. coli TG1 cells to produce fusion peptide, peptides were purified by CM sepharose ion-exchange column. In vitro bactericidal assays were made to identify the bioactivity of fusion peptide. RESULTS: Fusion peptide presented a specific bactericidal activity which was over one hundred times as effective as that of penicillin/oxacillin against tested Staphylococcus aureus strains. CONCLUSION: Fusion peptide behaved with a targeting bactericidal activity against Staphylococcus aureus which was lacking at two precursors, Staphylococcal pheromone and colicin Ia pore-forming region. These results suggest that an engineered multidomain protein machine with specific bactericidal activity has been constructed in the present study"
Keywords:		"Anti-Bacterial Agents/*biosynthesis Bacterial Proteins/*genetics Colicins/*genetics Genes, Bacterial Methicillin Resistance/*genetics Mutagenesis, Site-Directed Penicillin Resistance/genetics *Peptides Peptides, Cyclic Recombinant Fusion Proteins/biosynth;"
Notes:		"MedlineLiao, Ying Zhang, Jie Lu, Xiaofeng Wan, Lin Li, Shengfu Chen, Gang Gao, Yang Qiu, Xiaoqing chi English Abstract Research Support, Non-U.S. Gov't China 2003/11/19 Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Oct; 34(4):605-9"