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Microb Biotechnol

Title:		Activity of bacteria isolated from bats against Pseudogymnoascus destructans in China
Author(s):		Li Z; Li A; Hoyt JR; Dai W; Leng H; Li Y; Li W; Liu S; Jin L; Sun K; Feng J;
Address:		"College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130018, China. Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, 2555 Jingyue Street, Changchun, 130117, China. Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun, 130024, China. Department of Biological Sciences, Virginia Polytechnic Institute, Blacksburg, VA, 24060, USA. College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, China. Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo, 454000, China"
Journal Title:		Microb Biotechnol
Year:		2022
Volume:		20210209
Issue:		2
Page Number:		469 - 481
DOI:		10.1111/1751-7915.13765
ISSN/ISBN:		1751-7915 (Electronic) 1751-7915 (Linking)
Abstract:		"White-nose syndrome, a disease that is caused by the psychrophilic fungus Pseudogymnoascus destructans, has threatened several North America bat species with extinction. Recent studies have shown that East Asian bats are infected with P. destructans but show greatly reduced infections. While several factors have been found to contribute to these reduced infections, the role of specific microbes in limiting P. destructans growth remains unexplored. We isolated three bacterial strains with the ability to inhibit P. destructans, namely, Pseudomonas yamanorum GZD14026, Pseudomonas brenneri XRD11711 and Pseudomonas fragi GZD14479, from bats in China. Pseudomonas yamanorum, with the highest inhibition score, was selected to extract antifungal active substance. Combining mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identified the active compound inhibiting P. destructans as phenazine-1-carboxylic acid (PCA), and the minimal inhibitory concentration (MIC) was 50.12 mug ml(-1) . Whole genome sequencing also revealed the existence of PCA biosynthesis gene clusters. Gas chromatography-mass spectrometry (GC-MS) analysis identified volatile organic compounds. The results indicated that 10 ppm octanoic acid, 100 ppm 3-tert-butyl-4-hydroxyanisole (isoprenol) and 100 ppm 3-methyl-3-buten-1-ol (BHA) inhibited the growth of P. destructans. These results support that bacteria may play a role in limiting the growth of P. destructans on bats"
Keywords:		Animals *Ascomycota/genetics Bacteria *Chiroptera/microbiology/physiology Pseudomonas;
Notes:		"MedlineLi, Zhongle Li, Aoqiang Hoyt, Joseph R Dai, Wentao Leng, Haixia Li, Yanfei Li, Wei Liu, Sen Jin, Longru Sun, Keping Feng, Jiang eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2021/02/10 Microb Biotechnol. 2022 Feb; 15(2):469-481. doi: 10.1111/1751-7915.13765. Epub 2021 Feb 9"