| Title: | The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene |
| Author(s): | Li CP; Wu DH; Huang SH; Meng M; Shih HT; Lai MH; Chen LJ; Jena KK; Hechanova SL; Ke TJ; Chiu TY; Tsai ZY; Chen GK; Tsai KC; Leu WM; |
| Address: | "Crop Science Division, Taiwan Agricultural Research Institute, Council of Agriculture, Taichung 41362, Taiwan. Department of Plant Protection, Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Chiayi 60044, Taiwan. Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan. Applied Zoology Division, Taiwan Agricultural Research Institute, Council of Agriculture, Taichung 41362, Taiwan. Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, Taiwan. Gene Identification and Validation (GIV) Laboratory, Rice Breeding Innovation Platform, International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines. School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, India. Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line 'TNG71-Bph45' was developed from the Oryza sativa japonica variety 'Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice" |
| Keywords: | "Animals Genes, Plant *Hemiptera/genetics Limonene *Oryza/genetics Plant Diseases/genetics Oryza nivara Tainan 11 (TN11) Tainung 71 (TNG71) antixenosis brown planthopper (BPH) integrated pest management (IPM) near-isogenic line (NIL) terpene synthase (TPS);" |
| Notes: | "MedlineLi, Charng-Pei Wu, Dong-Hong Huang, Shou-Horng Meng, Menghsiao Shih, Hsien-Tzung Lai, Ming-Hsin Chen, Liang-Jwu Jena, Kshirod K Hechanova, Sherry Lou Ke, Ting-Jyun Chiu, Tai-Yuan Tsai, Zong-Yuan Chen, Guo-Kai Tsai, Kuan-Chieh Leu, Wei-Ming eng MOST 107-2321-B-005-012; MOST 108-2321-B-005-007; MOST 109-2321-B-005-023; and MOST 110-2321-B-005-011/the National Science and Technology Council of Taiwan/ Switzerland 2023/01/22 Int J Mol Sci. 2023 Jan 16; 24(2):1798. doi: 10.3390/ijms24021798" |
