Title: | Development of a bioluminescence assay for aldehyde pheromones of insects : I. Sensitivity and specificity |
Author(s): | Meighen EA; Slessor KN; Grant GG; |
Address: | "Department of Biochemistry, McGill University, 3655 Drummond Street, H3G 1Y6, Montreal, Quebec, Canada" |
ISSN/ISBN: | 0098-0331 (Print) 0098-0331 (Linking) |
Abstract: | "A rapid, quantitative assay for long-chain aldehydes based on bacterial luminescence was developed. Significant luminescent responses were obtained with both saturated and unsaturated aldehydes of 12-18 carbons. Maximum responses were obtained with the 14- and 16-carbon compounds, including those that are known insect sex pheromones. The bioluminescent response was linearly related to the amount of aldehyde over a 10(4)-10(5) concentration range with as little as 0.1 pmol ( approximately 20 pg) of aldehyde being detected. The bioluminescent assay represents a new quantitative tool for rapidly measuring aldehyde pheromones of insects" |
Notes: | "PubMed-not-MEDLINEMeighen, E A Slessor, K N Grant, G G eng 1982/06/01 J Chem Ecol. 1982 Jun; 8(6):911-21. doi: 10.1007/BF00987658" |