| Title: | HS-SPME coupled with GC-MS for elucidating differences between the volatile components in wild and cultivated Atractylodes chinensis |
| Author(s): | Lei H; Yue J; Yin XY; Fan W; Tan SH; Qin L; Zhao YN; Bai JH; |
| Address: | "Department of Biology, Xinzhou Teachers University, Xinzhou, China" |
| ISSN/ISBN: | 1099-1565 (Electronic) 0958-0344 (Linking) |
| Abstract: | "INTRODUCTION: Atractylodes chinensis is a Chinese herb that is used in traditional medicine; it contains volatile components that have enormous potential for pharmaceutical, food, and cosmetic applications. The destruction of wild resources demands significant improvement in the quality of artificial cultivation of Atractylodes chinensis. However, little is known about the compositional differences in the volatile substances derived from the wild and cultivated varieties of Atractylodes chinensis. OBJECTIVES: We aimed to evaluate the specific components of Atractylodes chinensis and analyse the similarities and differences between the volatile components and metabolic pathways in the wild and cultivated varieties. MATERIAL AND METHODS: Metabolomic analysis using gas chromatography-mass spectrometry (GC-MS) was employed following the extraction of volatile components from Atractylodes chinensis using headspace solid-phase microextraction (HS-SPME). RESULTS: A total of 167 volatile metabolites were extracted, and 137 substances were matched with NIST and Wiley databases. Among them, 76 compounds exhibited significant differences between the two sources; these mainly included terpenes, aromatics, and esters. KEGG enrichment analysis indicated that the differential metabolites were primarily involved in the biosynthesis of secondary metabolites, terpene biosynthesis, and limonene and pinene degradation; all these pathways have geranyl diphosphate (GDP) as the common link. CONCLUSION: The total content of volatile substances extracted from wild Atractylodes chinensis was 2.5 times higher than that from the cultured variety; however, each source had different dominant metabolites. This study underscores the necessity for protecting wild Atractylodes chinensis resources, while enhancing the quality of cultivated Atractylodes chinensis" |
| Keywords: | *Atractylodes Gas Chromatography-Mass Spectrometry/methods Solid Phase Microextraction/methods Terpenes Limonene/analysis *Volatile Organic Compounds/analysis Atractylodes chinensis artificial cultivation volatile metabolites wild material; |
| Notes: | "MedlineLei, Hui Yue, Jiao Yin, Xin-Yu Fan, Wei Tan, Song-Hui Qin, Li Zhao, Yi-Nian Bai, Jian-Hua eng 047/00000337/Research project commissioned by enterprises and public institutions of Xinzhou Normal University/ England 2023/01/25 Phytochem Anal. 2023 Apr; 34(3):317-328. doi: 10.1002/pca.3210. Epub 2023 Jan 23" |
