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Mol Cells

Title:		Molecular identification of Adoxophyes honmai (Yasuda) (Lepidoptera: Tortricidae) based on mitochondrial COI gene sequences
Author(s):		Lee SY; Park H; Boo KS; Park KT; Cho S;
Address:		"Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea"
Journal Title:		Mol Cells
Year:		2005
Volume:		19
Issue:		3
Page Number:		391 - 397
DOI:
ISSN/ISBN:		1016-8478 (Print) 1016-8478 (Linking)
Abstract:		"Molecular identification techniques are used where morphological characters are not useful for distinguishing species that resemble each other closely. The example studied here is the Adoxophyes species complex, in which A. orana (Fischer von Rosslerstamm) is officially the only known Korean species in the genus Adoxophyes (Lepidoptera: Tortricidae). However there have been suspicions that at least two types of A. orana exist in Korea based on the distribution and range of the host, with A. orana attacking apples and peaches, and another Adoxophyes sp. attacking tea and pears. The latter is presumed to be A. honmai (Yasuda), but the two have remained confused because of their extreme morphological similarity, despite several Asian studies of pheromonal and morphological characteristics. To confirm the occurrence of an Adoxophyes species other than A. orana in Korea, we compared 940 bp of the mitochondrial cytochrome oxidase I (COI) gene from 16 samples of Adoxophyes and found that there is a second Adoxophyes species different from A. orana. Comparison of the different sequences to that of Japanese A. honmai confirmed that they belong to the latter. From the sequence difference between the two Korean species, we were able to develop new PCR primer sets that distinguish them. This molecular identification technique with no enzyme digestion or sequencing step is a convenient and rapid way of differentiating between species that are hard to distinguish morphologically"
Keywords:		Animals Base Sequence DNA/chemistry Electron Transport Complex IV/*genetics Female Male Mitochondria/*enzymology/*genetics Molecular Sequence Data Moths/*classification/*genetics Polymerase Chain Reaction;
Notes:		"MedlineLee, So Young Park, Hyungjin Boo, Kyung Saeng Park, Kyu-Tek Cho, Soowon eng Research Support, Non-U.S. Gov't Korea (South) 2005/07/05 Mol Cells. 2005 Jun 30; 19(3):391-7"