| Title: | Development of supercritical CO(2) extraction of bioactive phytochemicals from black poplar (Populus nigra L.) buds followed by GC-MS and UHPLC-DAD-QqTOF-MS |
| Author(s): | Kus PM; Okinczyc P; Jakovljevic M; Jokic S; Jerkovic I; |
| Address: | "Department of Pharmacognosy, Wroclaw Medical University, ul. Borowska 211a, 50-556, Wroclaw, Poland. Electronic address: piotr.kus@umed.wroc.pl. Department of Pharmacognosy, Wroclaw Medical University, ul. Borowska 211a, 50-556, Wroclaw, Poland. Electronic address: piotrokinczyc@gmail.com. Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhaca 20, 31000, Osijek, Croatia. Electronic address: mjakovljevic@ptfos.hr. Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhaca 20, 31000, Osijek, Croatia. Electronic address: stela.jokic@ptfos.hr. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, R. Boskovica 35, 21000, Split, Croatia. Electronic address: igor@ktf-split.hr" |
| DOI: | 10.1016/j.jpba.2018.05.041 |
| ISSN/ISBN: | 1873-264X (Electronic) 0731-7085 (Linking) |
| Abstract: | "The supercritical CO(2) (SC-CO(2)) extraction process of black poplar (Populus nigra L.) buds was optimized (pressure, temperature) based on the yields of major phytochemicals (volatiles and non-volatiles). The optimal settings were 30?ª+MPa/60?ª+ degrees C. Major volatiles determined by GC-MS in the optimized SC-CO(2) extract (mg of benzyl salicylate equivalent (BSE) per 100?ª+g of buds) were: pinostrobin chalcone (1574.2), beta-eudesmol (640.8), alpha-eudesmol (581.9), 2-methyl-2-butenyl-p-coumarate (289.9), pentyl-p-coumarate (457.0), gamma-eudesmol (294.4), and benzyl salicylate (289.2). Partial qualitative similarity was observed between SC-CO(2) extracts and corresponding hydrodistilled essential oil dominated by sesquiterpenes, but with lower yields. Major compounds (mg per 100?ª+g of buds) identified by UHPLC-DAD-QqTOF-MS in the optimized SC-CO(2) extract were: pinostrobin (751.7), pinocembrin (485.6), 3-O-pinobanksin acetate and methyl-butenyl-p-coumarate (290.2; 144.9 of pinobanksin and p-coumaric acid equivalents, respectively). SC-CO(2) extraction was found useful for green, efficient and simultaneous extraction of both volatile/non-volatile, bioactive phytochemicals of poplar buds - precursors of poplar-type propolis" |
| Keywords: | "Carbon Dioxide/*chemistry Chemical Fractionation/instrumentation/*methods Chromatography, High Pressure Liquid/instrumentation/methods Flavonoids/isolation & purification/pharmacology Gas Chromatography-Mass Spectrometry/instrumentation/methods Green Chem;" |
| Notes: | "MedlineKus, Piotr M Okinczyc, Piotr Jakovljevic, Martina Jokic, Stela Jerkovic, Igor eng England 2018/06/01 J Pharm Biomed Anal. 2018 Sep 5; 158:15-27. doi: 10.1016/j.jpba.2018.05.041. Epub 2018 May 25" |
