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MethodsX

Title:		Development of an enzyme immunoassay to measure urinary and faecal 5alpha-androst-16-en-3-one in pigs
Author(s):		Kumar V; Umapathy G;
Address:		"Laboratory for the Conservation of Endangered Species (LaCONES), CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, Telangana 500048, India"
Journal Title:		MethodsX
Year:		2023
Volume:		20230413
Issue:
Page Number:		102178 -
DOI:		10.1016/j.mex.2023.102178
ISSN/ISBN:		2215-0161 (Print) 2215-0161 (Electronic) 2215-0161 (Linking)
Abstract:		"Androstenone, a volatile steroid that possesses pheromonal activity, is responsible for boar taint, sexual interactions, and reproduction in pigs. A wide range of analytical methods has been developed to quantify and detect androstenone in adipose tissue and blood, which are invasive procedures. Therefore, the present study aimed to develop a non-invasive method to detect and quantify the androstenone. We produced group-specific polyclonal androstenone antibody to standardize and validate an enzyme immunoassay to measure faecal and urinary androstenone in Yorkshire boars and sows. Parallelism was performed to determine the immunoreactivity between faecal and urinary immunoreactive androstenone and respective antibody. In boars, urinary and faecal androstenone concentrations were higher on the day of mounting and copulation with sows. In sows, we also measured faecal progesterone metabolites to confirm the oestrus and mating. Faecal androstenone concentrations were peaked on the day of oestrus and mating in sows. Our results suggest that androstenone could be detected and quantified in faecal and urine samples of boars and sows. *Developed an enzyme immunoassay for measuring 5alpha-androst-16-en-3-one as a marker of boar taint and sex pheromone in urine and faeces of pigs*Detection of 5alpha-androst-16-en-3-one using a non-invasive method"
Keywords:		Androstenone Antibody production Boar taint Development of an enzyme immunoassay for detection of faecal and urinary 5alpha-androst-16-en-3-one in pigs Enzyme immunoassay Faeces Pheromone Urine;
Notes:		"PubMed-not-MEDLINEKumar, Vinod Umapathy, Govindhaswamy eng Netherlands 2023/05/01 MethodsX. 2023 Apr 13; 10:102178. doi: 10.1016/j.mex.2023.102178. eCollection 2023"