| Title: | "Effects of juvenile hormone on gene expression in the pheromone-producing midgut of the pine engraver beetle, Ips pini" |
| Author(s): | Keeling CI; Bearfield JC; Young S; Blomquist GJ; Tittiger C; |
| Address: | "Department of Biochemistry and Molecular Biology, University of Nevada, Reno, USA. ckeeling@mac.com" |
| DOI: | 10.1111/j.1365-2583.2006.00629.x |
| ISSN/ISBN: | 0962-1075 (Print) 0962-1075 (Linking) |
| Abstract: | "Juvenile hormone III (JH III) stimulates biosynthesis of the monoterpenoid aggregation pheromone component, ipsdienol, in the anterior midgut of the male pine engraver beetle, Ips pini (Say). To understand better the hormonal regulation of pheromone biosynthesis in this forest pest, and identify JH III-responsive genes, microarrays were prepared and hybridized to cDNA from midguts of JH III-treated beetles. Expression patterns were confirmed by quantitative real-time RT-PCR. JH III co-ordinately regulated mevalonate pathway genes and many other genes implicated in pheromone biosynthesis. Sex differences in basal levels of mevalonate pathway genes were consistent with their role in male-specific pheromone biosynthesis. This is the first microarray-based study of the developmental and hormonal regulation of insect pheromone biosynthesis" |
| Keywords: | Animals Coleoptera/*metabolism Female Gastrointestinal Tract/metabolism Gene Expression Male Oligonucleotide Array Sequence Analysis Pheromones/*biosynthesis/genetics Reverse Transcriptase Polymerase Chain Reaction Sesquiterpenes/*metabolism; |
| Notes: | "MedlineKeeling, C I Bearfield, J C Young, S Blomquist, G J Tittiger, C eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2006/04/28 Insect Mol Biol. 2006 Apr; 15(2):207-16. doi: 10.1111/j.1365-2583.2006.00629.x" |
