| Title: | Inhibition of protein translocation in permeabilized cells of Schizosaccharomyces pombe by puromycin |
| Author(s): | Kambe-Honjoh H; Yoda K; Yamasaki M; |
| Address: | "Department of Agricultural Chemistry, University of Tokyo, Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 0916-8451 (Print) 0916-8451 (Linking) |
| Abstract: | "To investigate protein translocation in eukaryotes, we reconstituted a protein translocation system using the permeabilized spheroplasts (P-cells) of the fission yeast Schizosaccharomyces pombe. The precursor of a sex pheromone of Saccharomyces cerevisiae, prepro-alpha-factor, was translocated across the endoplasmic reticulum (ER) of S. pombe posttranslationally, and glycosylated to the same extent as in the ER of S. cerevisiae. This suggested that the size of N-linked core-oligosaccharide in the ER of S. pombe is similar to that in S. cerevisiae. This translocation into the ER of S. pombe was inhibited by puromycin, but the translocation in the P-cells of S. cerevisiae was not inhibited. This difference in sensitivity to puromycin was due to the membrane but not the cytosolic fraction. Our results suggested that the translocation machinery of S. pombe was sensitive to puromycin and different from that of S. cerevisiae" |
| Keywords: | "Cell Membrane Permeability/physiology Cytosol/metabolism/physiology Fungal Proteins/*genetics/metabolism Glycosylation Kinetics Protein Precursors/*genetics/metabolism Protein Processing, Post-Translational Puromycin/*pharmacology Ribonucleases/pharmacolo;" |
| Notes: | "MedlineKambe-Honjoh, H Yoda, K Yamasaki, M eng Research Support, Non-U.S. Gov't England 1992/10/01 Biosci Biotechnol Biochem. 1992 Oct; 56(10):1649-54. doi: 10.1271/bbb.56.1649" |
