| Title: | Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria |
| Author(s): | Junger M; Vautz W; Kuhns M; Hofmann L; Ulbricht S; Baumbach JI; Quintel M; Perl T; |
| Address: | "Department of Anesthesiology, Emergency and Intensive Care Medicine, University of Gottingen, Gottingen, Germany. melanie.juenger@med.uni-goettingen.de" |
| Journal Title: | Appl Microbiol Biotechnol |
| DOI: | 10.1007/s00253-012-3924-4 |
| ISSN/ISBN: | 1432-0614 (Electronic) 0175-7598 (Print) 0175-7598 (Linking) |
| Abstract: | "Presently, 2 to 4 days elapse between sampling at infection suspicion and result of microbial diagnostics. This delay for the identification of pathogens causes quite often a late and/or inappropriate initiation of therapy for patients suffering from infections. Bad outcome and high hospitalization costs are the consequences of these currently existing limited pathogen identification possibilities. For this reason, we aimed to apply the innovative method multi-capillary column-ion mobility spectrometry (MCC-IMS) for a fast identification of human pathogenic bacteria by determination of their characteristic volatile metabolomes. We determined volatile organic compound (VOC) patterns in headspace of 15 human pathogenic bacteria, which were grown for 24 h on Columbia blood agar plates. Besides MCC-IMS determination, we also used thermal desorption-gas chromatography-mass spectrometry measurements to confirm and evaluate obtained MCC-IMS data and if possible to assign volatile compounds to unknown MCC-IMS signals. Up to 21 specific signals have been determined by MCC-IMS for Proteus mirabilis possessing the most VOCs of all investigated strains. Of particular importance is the result that all investigated strains showed different VOC patterns by MCC-IMS using positive and negative ion mode for every single strain. Thus, the discrimination of investigated bacteria is possible by detection of their volatile organic compounds in the chosen experimental setup with the fast and cost-effective method MCC-IMS. In a hospital routine, this method could enable the identification of pathogens already after 24 h with the consequence that a specific therapy could be initiated significantly earlier" |
| Keywords: | Bacteria/chemistry/*isolation & purification/metabolism/pathogenicity Bacterial Infections/diagnosis/*microbiology Humans Metabolome Spectrum Analysis/*methods Volatile Organic Compounds/*analysis/metabolism; |
| Notes: | "MedlineJunger, Melanie Vautz, Wolfgang Kuhns, Martin Hofmann, Lena Ulbricht, Siobhan Baumbach, Jorg Ingo Quintel, Michael Perl, Thorsten eng Evaluation Study Research Support, Non-U.S. Gov't Germany 2012/02/14 Appl Microbiol Biotechnol. 2012 Mar; 93(6):2603-14. doi: 10.1007/s00253-012-3924-4. Epub 2012 Feb 12" |
