| Title: | Functional phylogenetics reveals contributions of pleiotropic peptide action to ligand-receptor coevolution |
| Author(s): | Jiang H; Wei Z; Nachman RJ; Adams ME; Park Y; |
| Address: | "1] Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China [2] Department of Entomology, Kansas State University, Manhattan, Kansas 66506, United States. Department of Entomology, Kansas State University, Manhattan, Kansas 66506, United States. Insect Control and Cotton Disease Research Unit, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College Station, TX 77845, USA. Departments of Entomology and Cell Biology &Neuroscience, 2103 Biological Sciences Building, University of California, Riverside, CA 92521, USA" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The evolution of peptidergic signaling has been accompanied by a significant degree of ligand-receptor coevolution. Closely related clusters of peptide signaling molecules are observed to activate related groups of receptors, implying that genes encoding these ligands may orchestrate an array of functions, a phenomenon known as pleiotropy. Here we examine whether pleiotropic actions of peptide genes might influence ligand-receptor coevolution. Four test groups of neuropeptides characterized by conserved C-terminal amino acid sequence motifs and their cognate receptors were examined in the red flour beetle (Tribolium castaneum): 1) cardioacceleratory peptide 2b (CAPA); CAPAr, 2) pyrokinin/diapause hormone (PK1/DH); PKr-A, -B, 3) pyrokinin/pheromone biosynthesis activating hormone (PK2/PBAN); PKr-C, and 4) ecdysis triggering hormone (ETH); ETHr-b. Ligand-receptor specificities were established through heterologous expression of receptors in cell-based assays for 9 endogenous ligands. Based on ligand-receptor specificity analysis, we found positive pleiotropism exhibited by ETH on ETHR-b and CAPAr, whereas PK1/DH and CAPA are more highly selective for their respective authentic receptors than would be predicted by phylogenetic analysis. Disparities between evolutionary trees deduced from receptor sequences vs. functional ligand-receptor specificities lead to the conclusion that pleiotropy exhibited by peptide genes influences ligand-receptor coevolution" |
| Keywords: | "Animals *Evolution, Molecular Ligands Neuropeptides/biosynthesis/*genetics/metabolism Oligopeptides/biosynthesis/*genetics Phylogeny Pyrrolidonecarboxylic Acid/*analogs & derivatives Signal Transduction Tribolium/genetics/metabolism;" |
| Notes: | "MedlineJiang, Hongbo Wei, Zhaojun Nachman, Ronald J Adams, Michael E Park, Yoonseong eng R01 AI090062/AI/NIAID NIH HHS/ R01AI090062/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2014/10/29 Sci Rep. 2014 Oct 28; 4:6800. doi: 10.1038/srep06800" |
