| Title: | Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor |
| Author(s): | Jenness DD; Burkholder AC; Hartwell LH; |
| DOI: | 10.1016/0092-8674(83)90186-1 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "The division cycle of yeast a cells is inhibited by alpha-factor. Haploid a cells were found to bind 35S-labeled alpha-factor, whereas haploid alpha cells and diploid a/alpha cells showed little binding. The association of alpha-factor with a cells was reversible upon dilution. Unlabeled alpha-factor competed for binding of 35S-alpha-factor; the concentration dependence for competition indicated 9 X 10(5) binding sites per cell with a dissociation constant (KD) of 3 X 10(-7) M. The rates of association (kon = 3 X 10(3) M-1 sec-1) and dissociation (koff = 9 X 10(-4) sec-1) were consistent with the equilibrium constant. The alpha-factor binding activity associated with five temperature-sensitive ste2 mutants was thermolabile, suggesting that the STE2 gene encodes the receptor for alpha-factor. In contrast, the binding activity of other temperature-sensitive mutants (ste4, ste5, ste7, ste11, and ste12) showed no thermolability" |
| Keywords: | Binding Sites Genotype Haploidy Kinetics Mating Factor Mutation Peptides/genetics/*metabolism Saccharomyces cerevisiae/*genetics Species Specificity Temperature; |
| Notes: | "MedlineJenness, D D Burkholder, A C Hartwell, L H eng Research Support, U.S. Gov't, P.H.S. 1983/12/01 Cell. 1983 Dec; 35(2 Pt 1):521-9. doi: 10.1016/0092-8674(83)90186-1" |
